Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle.
Amino acids in the uterine luminal fluid reflects the temporal changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle.
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DOI：
10.1371/journal.pone.0100010
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Lonergan P
中科院分区：
文献类型：
--
作者：
Forde N;Simintiras CA;Sturmey R;Mamo S;Kelly AK;Spencer TE;Bazer FW;Lonergan P
在牛身上，胎盘和母体的相互作用对妊娠的建立和维持至关重要。这种早期相互作用的一个主要组成部分涉及子宫上皮细胞营养物质的运输和关键分子的分泌，以帮助支持妊娠围着床期的胚胎发育。目的：1)分析牛发情周期子宫腔液中氨基酸(AA)含量的时间变化；2)了解妊娠引起的AA含量变化；3)确定AA转运蛋白在子宫内膜和胚胎中的表达；4)确定孕酮P4对这些转运蛋白的调节作用。妊娠母牛的天冬氨酸、精氨酸、谷氨酰胺、组氨酸、赖氨酸、异亮氨酸、亮氨酸、苯丙氨酸和酪氨酸的浓度在发情周期第16天下降，在第19天上升(P<；0.05)。谷氨酸只在怀孕母牛第19天升高(P<；0.001)。与妊娠小母牛相比，妊娠小母牛第7天UF中天冬酰胺浓度更高(P<；0.05)，而妊娠小母牛第16天Valine浓度更高(P<；0.05)。阳离子AA转运蛋白SLC7A1、SLC7A4和SLC7A6在动情周期/早孕子宫内膜中的表达与受孕期SLC7A4、SLC7A2和SLC7A1的表达变化相一致(P<；0.05)。子宫内膜中只有一种酸性氨基酸转运蛋白(SLC1A5)表达增加，而胚胎中SLC1A4的表达增加(P<；0.05)。中性AA转运蛋白SLC38A2和SLC7A5在子宫内膜中的表达呈一过性增加，而胚胎中SLC38A7、SLC43A2、SLC38A11和SLC7A8的表达也增加(P<；0.05)。P4下调SLC1A1、-1A4、-1A5、-38A2、-38A4、-38A7、-43A2、-6A14、-7A1、-7A5和-7A7在子宫内膜中的表达。结果表明，URF中AA的时间变化反映了黄牛妊娠早期子宫内膜和胚胎中转运蛋白表达的变化，其中一些表达被P4修饰。
In cattle, conceptus-maternal interactions are critical for the establishment and maintenance of pregnancy. A major component of this early interaction involves the transport of nutrients and secretion of key molecules by uterine epithelial cells to help support conceptus development during the peri-implantation period of pregnancy. Objectives were to: 1) analyze temporal changes in the amino acid (AA) content of uterine luminal fluid (ULF) during the bovine estrous cycle; 2) understand conceptus-induced alterations in AA content; 3) determine expression of AA transporters in the endometrium and conceptus; and 4) determine how these transporters are modulated by (Progesterone) P4. Concentrations of aspartic acid, arginine, glutamine, histidine, lysine, isoleucine, leucine, phenylalanine and tyrosine decreased on Day 16 of the estrous cycle but increased on Day 19 in pregnant heifers (P<0.05). Glutamic acid only increased in pregnant heifers on Day 19 (P<0.001). Asparagine concentrations were greater in ULF of cyclic compared to pregnant heifers on Day 7 (P<0.05) while valine concentrations were higher in pregnant heifers on Day 16 (P<0.05). Temporal changes in expression of the cationic AA transporters SLC7A1 SLC7A4 and SLC7A6 occurred in the endometrium during the estrous cycle/early pregnancy coordinate with changes in conceptus expression of SLC7A4, SLC7A2 and SLC7A1 (P<0.05). Only one acidic AA transporter (SLC1A5) increased in the endometrium while conceptus expression of SLC1A4 increased (P<0.05). The neutral AA transporters SLC38A2 and SLC7A5 increased in the endometrium in a temporal manner while conceptus expression of SLC38A7, SLC43A2, SLC38A11 and SLC7A8 also increased (P<0.05). P4 modified the expression of SLC1A1, -1A4, -1A5, -38A2, -38A4, -38A7, -43A2, -6A14, -7A1, -7A5 and -7A7 in the endometrium. Results demonstrate that temporal changes in AA in the ULF reflect changes in transporter expression in the endometrium and conceptus during early pregnancy in cattle, some of which are modified by P4.
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DOI：
10.1530/jrf.0.0590205
发表时间：
1980-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BETTERIDGE, KJ;EAGLESOME, MD;MITCHELL, D
通讯作者：
MITCHELL, D
影响因子：
3.8
作者：
Gray, CA;Burghardt, RC;Spencer, TE
通讯作者：
Spencer, TE
影响因子：
3.3
作者：
BAZER, FW
通讯作者：
BAZER, FW
影响因子：
3.8
作者：
Groebner, Anna E.;Rubio-Aliaga, Isabel;Ulbrich, Susanne E.
通讯作者：
Ulbrich, Susanne E.
影响因子：
3.6
作者：
Forde, N.;Carter, F.;Roche, J. F.
通讯作者：
Roche, J. F.