A Role of the FUZZY ONIONS LIKE Gene in Regulating Cell Death and Defense in Arabidopsis.
A Role of the FUZZY ONIONS LIKE Gene in Regulating Cell Death and Defense in Arabidopsis.
复制标题
拟南芥中模糊洋葱样基因在调节细胞死亡和防御中的作用
DOI：
10.1038/srep37797
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发表时间：
2016-11-29
影响因子：
4.6
通讯作者：
Lu H
中科院分区：
文献类型：
--
作者：
Tremblay A;Seabolt S;Zeng H;Zhang C;Böckler S;Tate DN;Duong VT;Yao N;Lu H
细胞程序性死亡（PCD）是许多生物体发育和对环境刺激反应的关键。已知酵母、苍蝇和哺乳动物中的模糊洋葱（FZO）蛋白影响线粒体融合和功能。拟南芥FZO-LIKE（FZL）是一种叶绿体蛋白，调节叶绿体形态和细胞死亡。我们克隆了FZL基因的基础上，病变模拟表型赋予的fzl突变。在这里，我们提供证据支持FZL已经进化出不同于其他生物同源物的新功能。我们发现fzl突变体对细菌病原菌假单胞菌和卵菌病原菌阿拉伯透明霜霉表现出增强的抗病性。除了改变叶绿体形态和细胞死亡，fzl显示活性氧（ROS）和自噬途径的激活。FZL和防御信号分子水杨酸在防御和细胞死亡控制中形成负反馈环。FZL不补充缺乏FZO 1基因的酵母菌株。总之，这些数据表明，拟南芥FZL基因是细胞死亡和抗病性的负调节因子，可能通过调节叶绿体中的ROS和自噬途径。
Programmed cell death (PCD) is critical for development and responses to environmental stimuli in many organisms. FUZZY ONIONS (FZO) proteins in yeast, flies, and mammals are known to affect mitochondrial fusion and function. Arabidopsis FZO-LIKE (FZL) was shown as a chloroplast protein that regulates chloroplast morphology and cell death. We cloned the FZL gene based on the lesion mimic phenotype conferred by an fzl mutation. Here we provide evidence to support that FZL has evolved new function different from its homologs from other organisms. We found that fzl mutants showed enhanced disease resistance to the bacterial pathogen Pseudomonas syringae and the oomycete pathogen Hyaloperonospora arabidopsidis. Besides altered chloroplast morphology and cell death, fzl showed the activation of reactive oxygen species (ROS) and autophagy pathways. FZL and the defense signaling molecule salicylic acid form a negative feedback loop in defense and cell death control. FZL did not complement the yeast strain lacking the FZO1 gene. Together these data suggest that the Arabidopsis FZL gene is a negative regulator of cell death and disease resistance, possibly through regulating ROS and autophagy pathways in the chloroplast.
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影响因子：
64.5
作者：
Chen H;Vermulst M;Wang YE;Chomyn A;Prolla TA;McCaffery JM;Chan DC
通讯作者：
Chan DC
影响因子：
64.5
作者：
Hofius, Daniel;Schultz-Larsen, Torsten;Petersen, Morten
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Westermann, Benedikt
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Mansfield, JW
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作者：
Friedrich, L;Lawton, K;Ryals, J
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Ryals, J