Dynamic changes in proprotein convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
Dynamic changes in proprotein convertase 2 activity in cortical neurons after ischemia/reperfusion and oxygen-glucose deprivation.
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缺血/再灌注和氧糖剥夺后皮质神经元前蛋白转化酶 2 活性的动态变化
DOI：
10.3969/j.issn.1673-5374.2013.01.011
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发表时间：
2013-01-05
影响因子：
6.1
通讯作者：
Yang T
中科院分区：
文献类型：
--
作者：
Zhan S;Zhou A;Piper C;Yang T
本研究采用大鼠大脑中动脉阻断100分钟建立短暂性局灶性脑缺血模型，并利用体外培养的大鼠皮质神经元建立实验性缺氧缺糖模型。随着再灌注时间的延长，缺血区皮质前蛋白转换酶2活性逐渐降低。在培养的大鼠皮质神经元中，随着缺氧-葡萄糖剥夺时间的延长，末端脱氧核苷酸转移酶介导的2‘-脱氧尿苷-5’-三磷酸-生物素缺口末端标记阳性神经元的数量显著增加，原蛋白转换酶2的活性也逐渐降低。这些实验结果表明，缺血大鼠皮质再灌流后原蛋白转换酶2活性降低，培养的大鼠皮质神经元缺氧缺糖后活性降低。这些酶活性的变化可能在脑损伤中起重要的病理作用。
In this study, a rat model of transient focal cerebral ischemia was established by performing 100 minutes of middle cerebral artery occlusion, and an in vitro model of experimental oxygen-glucose deprivation using cultured rat cortical neurons was established. Proprotein convertase 2 activity gradually decreased in the ischemic cortex with increasing duration of reperfusion. In cultured rat cortical neurons, the number of terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate-biotin nick end labeling-positive neurons significantly increased and proprotein convertase 2 activity also decreased gradually with increasing duration of oxygen-glucose deprivation. These experimental findings indicate that proprotein convertase 2 activity decreases in ischemic rat cortex after reperfusion, as well as in cultured rat cortical neurons after oxygen-glucose deprivation. These changes in enzyme activity may play an important pathological role in brain injury.
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影响因子：
--
作者：
BLOOMQUIST, BT;EIPPER, BA;MAINS, RE
通讯作者：
MAINS, RE
影响因子：
4.8
作者：
FRIEDMAN, TC;LOH, YP;BIRCH, NP
通讯作者：
BIRCH, NP
影响因子：
3.1
作者：
SEIDAH, NG;GASPAR, L;CHRETIEN, M
通讯作者：
CHRETIEN, M
DOI：
10.1073/pnas.94.13.6646
发表时间：
1997-06-24
影响因子：
11.1
作者：
Furuta, M;Yano, H;Steiner, DF
通讯作者：
Steiner, DF
影响因子：
15.9
作者：
Ni, XP;Pearce, D;Humphreys, MH
通讯作者：
Humphreys, MH