Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis.
Identification, cloning and characterization of sis7 and sis10 sugar-insensitive mutants of Arabidopsis.
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DOI：
10.1186/1471-2229-8-104
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发表时间：
2008-10-14
影响因子：
5.3
通讯作者：
Gibson SI
中科院分区：
文献类型：
--
作者：
Huang Y;Li CY;Biddle KD;Gibson SI
可溶性糖的水平，如葡萄糖和蔗糖，有助于调节许多植物的代谢、生理和发育过程。基因筛查正在帮助识别与植物糖反应有关的一些基因座，并揭示糖和植物激素反应途径之间的广泛相互作用。采用正向遗传筛选的方法，筛选出对高水平外源糖抑制拟南芥早期幼苗发育的抗性增强的突变体。报道了两个糖不敏感(Sis)突变体的位置克隆和鉴定，这两个突变体都与脱落酸(ABA)的生物合成或反应有关。携带SIS7/NCED3/STO1或SIS10/ABI3突变的植物对高水平外源GLC和Suc的抑制作用具有抗性。定量RT-PCR分析表明，在野生型萌发种子中，高浓度的GLC上调了ABA生物合成基因的转录水平。基因表达谱显示，在萌发的sis7-1/nced3-4/sto1-4种子中，有大量表达水平低于野生型种子的基因与生长素的生物合成或运输有关，这表明ABA和生长素反应途径之间存在相互作用。在种子萌发过程中，sisi10/abi3不同突变体幼苗的糖不敏感程度与其对ABA的不敏感程度呈极显著正相关。SIS7/NCED3/STO1基因的突变是干旱条件下ABA生物合成所必需的，它赋予了糖不敏感的表型，表明在ABA生物合成中的结构性作用不是授予糖不敏感所必需的。这里提出的研究结果清楚地表明，ABI3的突变可以赋予糖不敏感的表型，并通过显示ABA和糖不敏感在不同的ABI3突变体中显示出强烈的正相关来帮助解释先前关于这一主题的混合报告。表达谱揭示了一种潜在的生长素代谢和运输的新调节，在ABA缺乏的突变体sis7-1/nced3-4/sto1-4中。
The levels of soluble sugars, such as glucose and sucrose, help regulate many plant metabolic, physiological and developmental processes. Genetic screens are helping identify some of the loci involved in plant sugar response and reveal extensive cross-talk between sugar and phytohormone response pathways. A forward genetic screen was performed to identify mutants with increased resistance to the inhibitory effects of high levels of exogenous sugars on early Arabidopsis seedling development. The positional cloning and characterization of two of these sugar insensitive (sis) mutants, both of which are also involved in abscisic acid (ABA) biosynthesis or response, are reported. Plants carrying mutations in SIS7/NCED3/STO1 or SIS10/ABI3 are resistant to the inhibitory effects of high levels of exogenous Glc and Suc. Quantitative RT-PCR analyses indicate transcriptional upregulation of ABA biosynthesis genes by high concentrations of Glc in wild-type germinating seeds. Gene expression profiling revealed that a significant number of genes that are expressed at lower levels in germinating sis7-1/nced3-4/sto1-4 seeds than in wild-type seeds are implicated in auxin biosynthesis or transport, suggesting cross-talk between ABA and auxin response pathways. The degree of sugar insensitivity of different sis10/abi3 mutant seedlings shows a strong positive correlation with their level of ABA insensitivity during seed germination. Mutations in the SIS7/NCED3/STO1 gene, which is primarily required for ABA biosynthesis under drought conditions, confer a sugar-insensitive phenotype, indicating that a constitutive role in ABA biosynthesis is not necessary to confer sugar insensitivity. Findings presented here clearly demonstrate that mutations in ABI3 can confer a sugar-insensitive phenotype and help explain previous, mixed reports on this topic by showing that ABA and sugar insensitivity exhibit a strong positive correlation in different abi3 mutants. Expression profiling revealed a potentially novel regulation of auxin metabolism and transport in an ABA deficient mutant, sis7-1/nced3-4/sto1-4.
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影响因子：
64.8
作者：
Blilou, I;Xu, J;Scheres, B
通讯作者：
Scheres, B
影响因子：
7.2
作者：
FINKELSTEIN, RR
通讯作者：
FINKELSTEIN, RR
影响因子：
11.6
作者：
GIRAUDAT, J;HAUGE, BM;GOODMAN, HM
通讯作者：
GOODMAN, HM
影响因子：
11.6
作者：
Jang, JC;Leon, P;Sheen, J
通讯作者：
Sheen, J
影响因子：
7.2
作者：
Deak, KI;Malamy, J
通讯作者：
Malamy, J