A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests.
A large proportion of P. falciparum isolates in the Amazon region of Peru lack pfhrp2 and pfhrp3: implications for malaria rapid diagnostic tests.
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DOI：
10.1371/journal.pone.0008091
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发表时间：
2010-01-25
期刊：
影响因子：
3.7
通讯作者：
Cheng Q
中科院分区：
文献类型：
--
作者：
Gamboa D;Ho MF;Bendezu J;Torres K;Chiodini PL;Barnwell JW;Incardona S;Perkins M;Bell D;McCarthy J;Cheng Q
疟疾快速诊断测试（RDTs）具有改善疟疾诊断的巨大潜力，并且在疟疾病例管理、控制和消除方面发挥着越来越大的作用。秘鲁与其他南美国家一道，正在采取行动，将疟疾快速诊断治疗作为全球艾滋病、结核病和疟疾基金支持的疟疾控制规划的组成部分。选择最合适的疟疾快速发展疗法对规划的成功至关重要。在秘鲁伊基托斯收集的9个显微镜检测阳性恶性疟原虫样本中，有8个使用hrp2检测rdt检测为阴性或弱阳性。这些样品通过PCR检测pfhrp2和pfhrp3及其侧翼基因的存在，并通过ELISA检测HRP蛋白的存在。为调查酶切缺失寄生虫的地理范围及其发生时间，对秘鲁亚马逊地区不同地区采集的148份显微镜检查阳性恶性疟原虫样本进行了回顾性研究。9株分离株中有8株缺乏pfhrp2和/或pfhrp3基因以及一个或两个侧翼基因，ELISA证实了HRP的缺失。回顾性研究显示，148份样本中分别有61份（41%）和103份（70%）缺少pfhrp2或pfhrp3基因，32份（21.6%）缺乏两种hrp基因。这是首次发现恶性疟原虫分离株缺乏pfhrp2和/或pfhrp3。在秘鲁亚马逊地区广泛分布的地区，缺乏pfhrp2和/或pfhrp3的不同寄生虫的高频率和广泛分布表明，针对HRP2的疟疾RDTs将无法在秘鲁疟疾流行地区检测到高比例的恶性疟原虫，不应使用。在本地区，应采用检测疟原虫LDH或醛缩酶的rdt和高质量显微镜技术进行疟疾诊断。迫切需要调查这些寄生虫在秘鲁及其邻国的数量和地理分布。
Malaria rapid diagnostic tests (RDTs) offer significant potential to improve the diagnosis of malaria, and are playing an increasing role in malaria case management, control and elimination. Peru, along with other South American countries, is moving to introduce malaria RDTs as components of malaria control programmes supported by the Global Fund for AIDS, TB and malaria. The selection of the most suitable malaria RDTs is critical to the success of the programmes. Eight of nine microscopy positive P. falciparum samples collected in Iquitos, Peru tested negative or weak positive using HRP2-detecting RDTs. These samples were tested for the presence of pfhrp2 and pfhrp3 and their flanking genes by PCR, as well as the presence of HRP proteins by ELISA. To investigate for geographic extent of HRP-deleted parasites and their temporal occurrence a retrospective study was undertaken on 148 microscopy positive P. falciparum samples collected in different areas of the Amazon region of Peru. Eight of the nine isolates lacked the pfhrp2 and/or pfhrp3 genes and one or both flanking genes, and the absence of HRP was confirmed by ELISA. The retrospective study showed that 61 (41%) and 103 (70%) of the 148 samples lacked the pfhrp2 or pfhrp3 genes respectively, with 32 (21.6%) samples lacking both hrp genes. This is the first documentation of P. falciparum field isolates lacking pfhrp2 and/or pfhrp3. The high frequency and wide distribution of different parasites lacking pfhrp2 and/or pfhrp3 in widely dispersed areas in the Peruvian Amazon implies that malaria RDTs targeting HRP2 will fail to detect a high proportion of P. falciparum in malaria-endemic areas of Peru and should not be used. RDTs detecting parasite LDH or aldolase and quality microscopy should be use for malaria diagnosis in this region. There is an urgent need for investigation of the abundance and geographic distribution of these parasites in Peru and neighbouring countries.
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DOI：
10.1083/jcb.103.4.1269
发表时间：
1986-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Howard RJ;Uni S;Aikawa M;Aley SB;Leech JH;Lew AM;Wellems TE;Rener J;Taylor DW
通讯作者：
Taylor DW
影响因子：
4.4
作者：
Jiang H;Yi M;Mu J;Zhang L;Ivens A;Klimczak LJ;Huyen Y;Stephens RM;Su XZ
通讯作者：
Su XZ
影响因子：
168.9
作者：
BEADLE, C;LONG, GW;HOFFMAN, SL
通讯作者：
HOFFMAN, SL
影响因子：
1.9
作者：
Bellia, PL
通讯作者：
Bellia, PL
影响因子：
9.4
作者：
Forney, JR;Magill, AJ;Gasser, RA
通讯作者：
Gasser, RA