Self-sorting of nonmuscle myosins IIA and IIB polarizes the cytoskeleton and modulates cell motility.
Self-sorting of nonmuscle myosins IIA and IIB polarizes the cytoskeleton and modulates cell motility.
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DOI：
10.1083/jcb.201705167
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发表时间：
2017-09-04
期刊：
影响因子：
--
通讯作者：
Svitkina TM
中科院分区：
文献类型：
--
作者：
Shutova MS;Asokan SB;Talwar S;Assoian RK;Bear JE;Svitkina TM
Copolymerization of nonmuscle myosins IIA and IIB followed by their differential turnover in stress fibers leads to self-sorting of IIA and IIB along the front–rear axis of the cell, thus producing a polarized actin cytoskeleton. Nonmuscle myosin II (NMII) is uniquely responsible for cell contractility and thus defines multiple aspects of cell behavior. To generate contraction, NMII molecules polymerize into bipolar minifilaments. Different NMII paralogs are often coexpressed in cells and can copolymerize, suggesting that they may cooperate to facilitate cell motility. However, whether such cooperation exists and how it may work remain unknown. We show that copolymerization of NMIIA and NMIIB followed by their differential turnover leads to self-sorting of NMIIA and NMIIB along the front–rear axis, thus producing a polarized actin–NMII cytoskeleton. Stress fibers newly formed near the leading edge are enriched in NMIIA, but over time, they become progressively enriched with NMIIB because of faster NMIIA turnover. In combination with retrograde flow, this process results in posterior accumulation of more stable NMIIB-rich stress fibers, thus strengthening cell polarity. By copolymerizing with NMIIB, NMIIA accelerates the intrinsically slow NMIIB dynamics, thus increasing cell motility and traction and enabling chemotaxis.
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DOI：
10.3791/2089
发表时间：
2010-08-10
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Cretu, Alexandra;Castagnino, Paola;Assoian, Richard
通讯作者：
Assoian, Richard
影响因子：
4
作者：
Kovac, Bianca;Teo, Jessica L.;Vallenius, Tea
通讯作者：
Vallenius, Tea
影响因子：
3.7
作者：
Betapudi V
通讯作者：
Betapudi V
影响因子：
3.4
作者：
Dembo, M;Wang, YL
通讯作者：
Wang, YL
影响因子：
11.2
作者：
Betapudi, Venkaiah;Licate, Lucila S.;Egelhoff, Thomas T.
通讯作者：
Egelhoff, Thomas T.