Mouse embryonic phenotyping by morphometric analysis of MR images.
Mouse embryonic phenotyping by morphometric analysis of MR images.
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DOI：
10.1152/physiolgenomics.00091.2010
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发表时间：
2010-10
影响因子：
4.6
通讯作者：
Sled JG
中科院分区：
文献类型：
--
作者：
Zamyadi M;Baghdadi L;Lerch JP;Bhattacharya S;Schneider JE;Henkelman RM;Sled JG
本文介绍了一种自动检测小鼠胚胎细微形态表型的新方法。基于高分辨率磁共振成像扫描和非线性图像对齐，该方法通过比较两个近交系C57 BL/6 J和129 Sv/S1 ImJ在受孕后15.5天的形态来证明。发现小鼠胚胎形态非常适合此类分析，在消除姿势和尺度的线性差异后，品系内的残留解剖变异水平非常低（平均110 μm）。局部大小差异的映射显示，C57 BL/6 J胚胎大于129 Sv/S1 ImJ胚胎，尽管这些差异在解剖结构上并不均匀分布。以器官体积表示，心脏和肺在C57 BL/6 J胚胎中较大，而脑和肝在品系之间的体积相当。器官大小和胚胎大小之间的正相关关系是一致的两个品系，但不同的器官，大脑和肝脏是最小的变量。总之，这些发现表明了这项技术在检测突变基因引起的细微表型差异方面的能力。
A new method is described for automatic detection of subtle morphological phenotypes in mouse embryos. Based on high-resolution magnetic resonance imaging scanning and nonlinear image alignment, this method is demonstrated by comparing the morphology of two inbred strains, C57BL/6J and 129Sv/S1ImJ, at 15.5 days postconception. Mouse embryo morphology was found to be highly amenable to this kind of analysis with very low levels (on average 110 μm) of residual anatomical variation within strains after linear differences in pose and scale are removed. Mapping of local size differences showed that C57BL/6J embryos were larger than 129Sv/S1ImJ embryos, although these differences were not uniformly distributed across the anatomy. Expressed in terms of organ volumes, heart and lung were larger in C57BL/6J embryos, while brain and liver were comparable in volume between strains. The positive relationship between organ size and embryo size was consistent for the two strains but differed by organ, with the brain and liver being the least variable. Together these findings suggest the power of this technique for detecting subtle phenotypic differences arising from mutated genes.
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影响因子：
10.6
作者：
Sled, JG;Zijdenbos, AP;Evans, AC
通讯作者：
Evans, AC
影响因子：
5.7
作者：
Genovese, CR;Lazar, NA;Nichols, T
通讯作者：
Nichols, T
影响因子：
5.7
作者：
Chung, MK;Worsley, KJ;Evanst, AC
通讯作者：
Evanst, AC
影响因子：
5.7
作者：
Spring, Shoshana;Lerch, Jason P.;Henkelman, R. Mark
通讯作者：
Henkelman, R. Mark
影响因子：
4.6
作者：
Nieman, BJ;Flenniken, AM;Sled, JG
通讯作者：
Sled, JG