Mechanism for the protective effect of resveratrol against oxidative stress-induced neuronal death.
Mechanism for the protective effect of resveratrol against oxidative stress-induced neuronal death.
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DOI：
10.1016/j.freeradbiomed.2010.06.002
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发表时间：
2010-09-01
影响因子：
7.4
通讯作者：
Zhu, Bao Ting
中科院分区：
文献类型：
--
作者：
Fukui, Masayuki;Choi, Hye Joung;Zhu, Bao Ting
氧化应激可诱导神经元细胞毒性，在神经元损伤和变性的病因学中起重要作用。本研究旨在确定白藜芦醇对氧化性神经元死亡的保护作用的细胞和生化机制。培养的HT 22细胞，一个永生化的小鼠海马神经元细胞系，被用作体外模型，这些神经元细胞中的氧化应激和神经毒性被暴露于高浓度的谷氨酸诱导。白藜芦醇强烈保护HT 22细胞免受谷氨酸诱导的氧化性细胞死亡。白藜芦醇的神经保护作用不依赖于其直接清除自由基的特性，而是依赖于其选择性诱导线粒体超氧化物歧化酶（SOD 2）表达，从而减少线粒体氧化应激和损伤的能力。白藜芦醇通过激活PI 3 K/Akt和GSK-3β/β-catenin信号通路诱导线粒体SOD 2的表达。总之，本研究的结果表明，白藜芦醇上调线粒体SOD 2是其保护神经细胞免受线粒体氧化应激引起的氧化细胞毒性的重要机制。
Oxidative stress can induce cytotoxicity in neurons, which plays an important role in the etiology of neuronal damage and degeneration. The present study seeks to determine the cellular and biochemical mechanisms underlying resveratrol’s protective effect against oxidative neuronal death. The cultured HT22 cells, an immortalized mouse hippocampal neuronal cell line, were used as an in vitro model, and the oxidative stress and neurotoxicity in these neuronal cells were induced by exposure to high concentrations of glutamate. Resveratrol strongly protected HT22 cells from glutamate-induced oxidative cell death. Resveratrol’s neuroprotective effect was independent of its direct radical-scavenging property, but instead was dependent on its ability to selectively induce the expression of mitochondrial superoxide dismutase (SOD2), and subsequently, reduce mitochondrial oxidative stress and damage. The induction of the mitochondrial SOD2 by resveratrol was mediated through the activation of the PI3K/Akt and GSK-3β/β-catenin signaling pathways. Taken together, the results of this study show that up-regulation of the mitochondrial SOD2 by resveratrol represents an important mechanism for its protection of neuronal cells against oxidative cytotoxicity resulting form mitochondrial oxidative stress.
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DOI：
10.1002/neu.480230915
发表时间：
1992-11-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
CHOI, DW
通讯作者：
CHOI, DW
影响因子：
4.8
作者：
Horovitz-Fried, M;Cooper, DR;Sampson, SR
通讯作者：
Sampson, SR
影响因子：
4.8
作者：
Connor, KM;Subbaram, S;Melendez, JA
通讯作者：
Melendez, JA
影响因子：
3.7
作者：
Alvarez, AR;Godoy, JA;Inestrosa, NC
通讯作者：
Inestrosa, NC
影响因子：
2.8
作者：
Gill, Catherine;Walsh, Sinead E.;Watson, R. William G.
通讯作者：
Watson, R. William G.