A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
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免疫沉淀级单克隆抗体的工具箱。
DOI：
10.1038/nmeth.4632
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发表时间：
2018-05
期刊：
影响因子：
48
通讯作者：
Blackshaw S
中科院分区：
文献类型：
--
作者：
Venkataraman A;Yang K;Irizarry J;Mackiewicz M;Mita P;Kuang Z;Xue L;Ghosh D;Liu S;Ramos P;Hu S;Bayron Kain D;Keegan S;Saul R;Colantonio S;Zhang H;Behn FP;Song G;Albino E;Asencio L;Ramos L;Lugo L;Morell G;Rivera J;Ruiz K;Almodovar R;Nazario L;Murphy K;Vargas I;Rivera-Pacheco ZA;Rosa C;Vargas M;McDade J;Clark BS;Yoo S;Khambadkone SG;de Melo J;Stevanovic M;Jiang L;Li Y;Yap WY;Jones B;Tandon A;Campbell E;Montelione GT;Anderson S;Myers RM;Boeke JD;Fenyö D;Whiteley G;Bader JS;Pino I;Eichinger DJ;Zhu H;Blackshaw S
努力解决生物医学研究中的重复性危机的一个关键组成部分是开发经过严格验证的可再生蛋白质亲和试剂。作为美国国立卫生研究院(NIH)蛋白质捕获试剂计划(PCRP)的一部分，我们使用集成的生产和验证流水线，产生了1,406株高度有效的针对737人转录因子的免疫沉淀和/或免疫印迹级小鼠单抗(MAbs)。我们使用HuProt人类蛋白芯片作为主要的验证工具来识别具有高度特异性的同源靶标单抗。我们通过免疫沉淀、免疫印迹、染色质免疫沉淀后测序(CHIP-SEQ)和免疫组织化学等多种实验应用进一步验证了PCRP单抗的有效性。我们还进行了一项荟萃分析，确定了有助于产生高质量单抗的关键变量。所有验证数据、方案和到PCRPmAb供应商的链接均可在http://proteincapture.org.上获得
A key component of efforts to address the reproducibility crisis in biomedical research is the development of rigorously validated and renewable protein-affinity reagents. As part of the US National Institutes of Health (NIH) Protein Capture Reagents Program (PCRP), we have generated a collection of 1,406 highly validated immunoprecipitation- and/or immunoblotting-grade mouse monoclonal antibodies (mAbs) to 737 human transcription factors, using an integrated production and validation pipeline. We used HuProt human protein microarrays as a primary validation tool to identify mAbs with high specificity for their cognate targets. We further validated PCRP mAbs by means of multiple experimental applications, including immunoprecipitation, immunoblotting, chromatin immunoprecipitation followed by sequencing (ChIP-seq), and immunohistochemistry. We also conducted a meta-analysis that identified critical variables that contribute to the generation of high-quality mAbs. All validation data, protocols, and links to PCRP mAb suppliers are available at http://proteincapture.org.
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影响因子：
5.3
作者：
Roncador G;Engel P;Maestre L;Anderson AP;Cordell JL;Cragg MS;Šerbec VČ;Jones M;Lisnic VJ;Kremer L;Li D;Koch-Nolte F;Pascual N;Rodríguez-Barbosa JI;Torensma R;Turley H;Pulford K;Banham AH
通讯作者：
Banham AH
DOI：
10.1073/pnas.1107488109
发表时间：
2012-03-20
影响因子：
11.1
作者：
de Melo, Jimmy;Miki, Katsuaki;Blackshaw, Seth
通讯作者：
Blackshaw, Seth
影响因子：
5.3
作者：
Rhodes, Kenneth J.;Trimmer, James S.
通讯作者：
Trimmer, James S.
影响因子：
2.9
作者：
Abdiche, Yasmina;Malashock, Dan;Pons, Jaurne
通讯作者：
Pons, Jaurne
影响因子：
7.7
作者：
Hu S;Wan J;Su Y;Song Q;Zeng Y;Nguyen HN;Shin J;Cox E;Rho HS;Woodard C;Xia S;Liu S;Lyu H;Ming GL;Wade H;Song H;Qian J;Zhu H
通讯作者：
Zhu H