Sendai virus-based liposomes enable targeted cytosolic delivery of nanoparticles in brain tumor-derived cells.
Sendai virus-based liposomes enable targeted cytosolic delivery of nanoparticles in brain tumor-derived cells.
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DOI：
10.1186/1477-3155-10-9
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发表时间：
2012-02-17
影响因子：
10.2
通讯作者：
Vazquez M
中科院分区：
文献类型：
--
作者：
Dudu V;Rotari V;Vazquez M
基于纳米技术的生物检测技术检测细胞标记组合的存在和/或缺失，越来越多地用于识别干细胞或祖细胞，评估细胞异质性，评估肿瘤恶性和/或化疗耐药性。使纳米颗粒能够快速检测活检细胞簇内新出现的细胞内标记物的递送方法将极大地有助于肿瘤表征、功能状态分析和治疗方案的开发。实验利用仙台病毒在体外实现了从人类脑肿瘤培养的细胞中量子点的细胞质递送。利用荧光显微镜和透射电子显微镜，体外实验表明，这些基于病毒的脂质体在研究的人类胶质母细胞瘤和髓母细胞瘤样本中，将非特异性内吞纳米颗粒的数量减少了50%。值得注意的是，基于病毒的脂质体递送也促进了量子点与培养细胞内细胞质表皮生长因子受体的靶向结合，这是恶性脑肿瘤早期检测和表征的重点。这些发现是首次利用仙台病毒在人脑肿瘤细胞内实现Qdots的胞浆性靶向胞内结合。这一结果对于纳米粒子继续适用于癌细胞的分子标记，以确定肿瘤的异质性、分级和化疗电阻率具有重要意义。
Nanotechnology-based bioassays that detect the presence and/or absence of a combination of cell markers are increasingly used to identify stem or progenitor cells, assess cell heterogeneity, and evaluate tumor malignancy and/or chemoresistance. Delivery methods that enable nanoparticles to rapidly detect emerging, intracellular markers within cell clusters of biopsies will greatly aid in tumor characterization, analysis of functional state and development of treatment regimens. Experiments utilized the Sendai virus to achieve in vitro, cytosolic delivery of Quantum dots in cells cultured from Human brain tumors. Using fluorescence microscopy and Transmission Electron Microscopy, in vitro experiments illustrated that these virus-based liposomes decreased the amount of non-specifically endocytosed nanoparticles by 50% in the Human glioblastoma and medulloblastoma samples studied. Significantly, virus-based liposome delivery also facilitated targeted binding of Quantum dots to cytosolic Epidermal Growth Factor Receptor within cultured cells, focal to the early detection and characterization of malignant brain tumors. These findings are the first to utilize the Sendai virus to achieve cytosolic, targeted intracellular binding of Qdots within Human brain tumor cells. The results are significant to the continued applicability of nanoparticles used for the molecular labeling of cancer cells to determine tumor heterogeneity, grade, and chemotherapeutic resistivity.
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影响因子：
17.1
作者：
Contreras J;Xie J;Chen YJ;Pei H;Zhang G;Fraser CL;Hamm-Alvarez SF
通讯作者：
Hamm-Alvarez SF
影响因子：
11.2
作者：
Galli, R;Binda, E;Vescovi, A
通讯作者：
Vescovi, A
靶向细胞外纳米颗粒可在活脑癌细胞中对活化表皮生长因子受体的细胞内检测。
影响因子：
5.4
作者：
Dudu, Veronica;Rotari, Veronica;Vazquez, Maribel
通讯作者：
Vazquez, Maribel
DOI：
10.1073/pnas.1011379108
发表时间：
2011-02-01
影响因子：
11.1
作者：
Dhar, Shanta;Kolishetti, Nagesh;Farokhzad, Omid C.
通讯作者：
Farokhzad, Omid C.
影响因子：
3.3
作者：
Cho, Der-Yang;Lin, Shinn-Zong;Chiu, Shao-Chih
通讯作者：
Chiu, Shao-Chih