Nonadhesive culture system as a model of rapid sphere formation with cancer stem cell properties.
Nonadhesive culture system as a model of rapid sphere formation with cancer stem cell properties.
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DOI：
10.1371/journal.pone.0031864
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin YS
中科院分区：
文献类型：
--
作者：
Chen SF;Chang YC;Nieh S;Liu CL;Yang CY;Lin YS
肿瘤干细胞在肿瘤的发生、发展和转移中起着重要作用，并且是导致高治疗失败率的原因。CSC的鉴定和表征对于促进癌症的监测、治疗或预防至关重要。为制定更有效的方法作出了巨大努力。尽管如此，CSC研究的理想模型仍在不断发展。在这项研究中，我们建立了一个非粘附培养系统，以丰富CSC从人口腔鳞状细胞癌细胞系与球形成，并进一步表征其CSC的特性。设计非粘附培养系统以从SAS和OECM-1细胞系产生球体。随后的调查，他们的CSC属性，包括干性，自我更新，和化疗和放射抗性在体外，以及在体内的肿瘤起始能力，也进行了。在5至7天内形成球体具有成本效益和时间效益。此外，我们证明，这些球表达假定的干细胞标记物，并表现出耐化疗，除了肿瘤启动和自我更新能力。使用这种非粘附培养系统，我们成功地建立了一个快速和具有成本效益的模型，表现出CSC的特性，并可用于癌症研究。
Cancer stem cells (CSCs) play an important role in tumor initiation, progression, and metastasis and are responsible for high therapeutic failure rates. Identification and characterization of CSC are crucial for facilitating the monitoring, therapy, or prevention of cancer. Great efforts have been paid to develop a more effective methodology. Nevertheless, the ideal model for CSC research is still evolving. In this study, we created a nonadhesive culture system to enrich CSCs from human oral squamous cell carcinoma cell lines with sphere formation and to characterize their CSC properties further. A nonadhesive culture system was designed to generate spheres from the SAS and OECM-1 cell lines. A subsequent investigation of their CSC properties, including stemness, self-renewal, and chemo- and radioresistance in vitro, as well as tumor initiation capacity in vivo, was also performed. Spheres were formed cost-effectively and time-efficiently within 5 to 7 days. Moreover, we proved that these spheres expressed putative stem cell markers and exhibited chemoradiotherapeutic resistance, in addition to tumor-initiating and self-renewal capabilities. Using this nonadhesive culture system, we successfully established a rapid and cost-effective model that exhibits the characteristics of CSCs and can be used in cancer research.
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影响因子：
11.5
作者：
Chiou, Shih-Hwa;Yu, Cheng-Chia;Lo, Jeng-Fan
通讯作者：
Lo, Jeng-Fan
DOI：
10.1016/j.bbrc.2009.05.048
发表时间：
2009-07-31
影响因子：
3.1
作者：
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通讯作者：
Lo, Wen-Liang
影响因子：
3.7
作者：
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通讯作者：
Chen CY
影响因子：
21.3
作者：
Avizienyte, E;Wyke, AW;Frame, MC
通讯作者：
Frame, MC
影响因子：
4
作者：
Bailey, Jennifer M.;Singh, Pankaj K.;Hollingsworth, Michael A.
通讯作者：
Hollingsworth, Michael A.