Glutathione revisited: a better scavenger than previously thought.
Glutathione revisited: a better scavenger than previously thought.
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DOI：
10.3389/fphar.2014.00260
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发表时间：
2014
影响因子：
5.6
通讯作者：
Bast A
中科院分区：
文献类型：
--
作者：
Haenen GR;Bast A
谷胱甘肽（GSH）是清除抗氧化剂的经典例子。它形成了第一道防线，并有效地清除活性物质，如次氯酸（HOCl），在它们对生物分子造成损害之前。清除抗氧化活性最好建立在竞争分析（密切模仿生物效应的分子机制）。在这种类型的分析中，抗氧化剂与一种分子竞争，这种分子作为一种易于读出的检测器来检测活性物质。一般认为，清除抗氧化活性反映了抗氧化剂与活性物质的反应速率常数（ka）。然而，对几种以HOCl为活性物质的谷胱甘肽竞争试验的批判性评价表明，ka不能决定清除抗氧化活性。比较了以乙酰胆碱酯酶、α - 1抗蛋白酶、蛋氨酸和白蛋白为检测手段的检测方法。被谷胱甘肽清除的活性物质的分子总数加上部分氧化形式的谷胱甘肽的分子总数反映了谷胱甘肽的清除活性。谷胱甘肽部分氧化形式的贡献取决于竞争分子的反应性。在一些试验中，部分氧化形式的谷胱甘肽对谷胱甘肽的清除活性有很大的贡献。与普遍的看法相反，反映GSH等抗氧化剂真实清除活性的不是反应速率，而是被清除的活性物质的分子总数。
Glutathione (GSH) is the classical example of a scavenging antioxidant. It forms the first line of defense and efficiently scavenges reactive species, e.g., hypochlorous acid (HOCl), before they inflict damage to biomolecules. Scavenging antioxidant activity is best established in competition assays (that closely mimics molecular mechanism of the biological effect). In this type of assay, the antioxidant competes with a molecule that functions as an easy read-out detector for a reactive species. It is generally assumed that the scavenging antioxidant activity reflects the reaction rate constant of the antioxidant with the reactive species (ka). However, critical appraisal of several competition assays of GSH with HOCl as reactive species, reveals that ka does not determine the scavenging antioxidant activity. Assays using acetylcholine esterase, alpha1-antiprotease, methionine, and albumin as detector are compared. The total number of molecules of the reactive species scavenged by GSH plus that by partially oxidized forms of the GSH, reflect the scavenging activity of GSH. The contribution of the partially oxidized forms of GSH depends on the reactivity of the competing molecule. In several assays the partially oxidized forms of GSH have a substantial contribution to the scavenging activity of GSH. In contrast to the prevailing perception, not the reaction rate but rather the total number of molecules of the reactive species scavenged reflects the true scavenging activity of an antioxidant like GSH.
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影响因子：
4.1
作者：
Winterbourn, CC;Brennan, SO
通讯作者：
Brennan, SO
影响因子：
3.8
作者：
den Hartog, GJM;Vegt, E;Bast, A
通讯作者：
Bast, A
影响因子：
7.4
作者：
Peskin, AV;Winterbourn, CC
通讯作者：
Winterbourn, CC
影响因子：
7.4
作者：
Balk, Jiska M.;Bast, Aalt;Haenen, Guido R. M. M.
通讯作者：
Haenen, Guido R. M. M.
影响因子：
3.7
作者：
Park HY;Man SF;Tashkin D;Wise RA;Connett JE;Anthonisen NA;Sin DD
通讯作者：
Sin DD