Direct current stimulation of endothelial monolayers induces a transient and reversible increase in transport due to the electroosmotic effect.
Direct current stimulation of endothelial monolayers induces a transient and reversible increase in transport due to the electroosmotic effect.
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DOI：
10.1038/s41598-018-27524-9
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发表时间：
2018-06-18
影响因子：
4.6
通讯作者：
Tarbell JM
中科院分区：
文献类型：
--
作者：
Cancel LM;Arias K;Bikson M;Tarbell JM
我们研究了直流电刺激(DC)在体外对内皮细胞(EC)单层液体和溶质转运的影响。我们的动机是经颅直流电刺激(TDC)，已被研究用于治疗神经精神障碍，增强神经康复，并改变健康受试者的认知。这种多样性应用背后的机制仍在调查中。为了解决血脑屏障(BBB)变化在tdcs过程中的可能作用，我们在一个特殊设计的室内对培养的EC单层施加直流电，该室产生空间均匀的直流电。DC诱导了流体和溶质在EC层之间的移动，这种移动只持续到刺激的持续时间，这表明了一种电渗透机制。诱导转运的方向与DCS的极性相反--这是电渗效应的一个标志。集散控制系统诱导的流量大小与外加电流的大小呈线性相关。基于内皮转运屏障的双孔描述和电双层的Helmholtz模型的数学模型准确地描述了实验数据，并预测了这一机制在渗透性较差的单层中的增强意义。本研究表明，dcs瞬时改变了血脑屏障的转运功能，提示了tdcs的一种新的辅助机制。
We investigated the effects of direct current stimulation (DCS) on fluid and solute transport across endothelial cell (EC) monolayers in vitro. Our motivation was transcranial direct current stimulation (tDCS) that has been investigated for treatment of neuropsychiatric disorders, to enhance neurorehabilitation, and to change cognition in healthy subjects. The mechanisms underlying this diversity of applications remain under investigation. To address the possible role of blood-brain barrier (BBB) changes during tDCS, we applied direct current to cultured EC monolayers in a specially designed chamber that generated spatially uniform direct current. DCS induced fluid and solute movement across EC layers that persisted only for the duration of the stimulation suggesting an electroosmosis mechanism. The direction of induced transport reversed with DCS polarity – a hallmark of the electroosmotic effect. The magnitude of DCS-induced flow was linearly correlated to the magnitude of the applied current. A mathematical model based on a two-pore description of the endothelial transport barrier and a Helmholtz model of the electrical double layer describes the experimental data accurately and predicts enhanced significance of this mechanism in less permeable monolayers. This study demonstrates that DCS transiently alters the transport function of the BBB suggesting a new adjunct mechanism of tDCS.
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影响因子：
7.7
作者：
Brunoni, Andre Russowsky;Nitsche, Michael A.;Bolognini, Nadia;Bikson, Marom;Wagner, Tim;Merabet, Lotfi;Edwards, Dylan J.;Valero-Cabre, Antoni;Rotenberg, Alexander;Pascual-Leone, Alvaro;Ferrucci, Roberta;Priori, Alberto;Boggio, Paulo Sergio;Fregni, Felipe
通讯作者：
Fregni, Felipe
DOI：
10.1039/c3ib40199e
发表时间：
2014-03
期刊：
Integrative biology : quantitative biosciences from nano to macro
影响因子：
--
作者：
Ebong EE;Lopez-Quintero SV;Rizzo V;Spray DC;Tarbell JM
通讯作者：
Tarbell JM
影响因子：
8.3
作者：
Chien, S;Li, S;Shyy, JYJ
通讯作者：
Shyy, JYJ
在经颅直流电流刺激过程中，同时测量脑血液动力学和脑电图。
影响因子：
5.3
作者：
Giovannella M;Ibañez D;Gregori-Pla C;Kacprzak M;Mitjà G;Ruffini G;Durduran T
通讯作者：
Durduran T
影响因子：
5.5
作者：
Jamil, Asif;Batsikadze, Giorgi;Nitsche, Michael A.
通讯作者：
Nitsche, Michael A.