bicaudal-C is required for the formation of anterior neurogenic ectoderm in the sea urchin embryo.
bicaudal-C is required for the formation of anterior neurogenic ectoderm in the sea urchin embryo.
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DOI：
10.1038/srep06852
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发表时间：
2014-10-31
影响因子：
4.6
通讯作者：
Inaba K
中科院分区：
文献类型：
--
作者：
Yaguchi S;Yaguchi J;Inaba K
bicaudal-C (bicC) mRNA编码一种含有rna结合结构域的蛋白质，据报道，这种蛋白质在某些物种的卵母细胞/卵子中存在偏转。在果蝇和青蛙的早期胚胎发生过程中，翻译的蛋白在沿着身体轴的细胞命运规范调节中起着关键作用。然而，目前尚不清楚它如何在bicC mRNA均匀分布的卵子中发挥作用，例如海胆卵。在这里，我们展示了BicC在海胆胚胎神经源性外胚层形成中的功能。功能缺失实验表明，BicC是5 -羟色胺能神经发生和ankAT-1基因表达所必需的，ankAT-1基因是海胆胚胎神经源性外胚层顶端绒毛形成所必需的。相比之下，神经源性外胚层特异转录因子FoxQ2的表达在BicC变体中是不变的。由于FoxQ2是5 -羟色胺能神经发生和ankAT-1表达的上游因子，这些数据表明BicC在海胆胚胎发生过程中调节由FoxQ2协调的事件。
bicaudal-C (bicC) mRNA encodes a protein containing RNA-binding domains that is reported to be maternally present with deflection in the oocytes/eggs of some species. The translated protein plays a critical role in the regulation of cell fate specification along the body axis during early embryogenesis in flies and frogs. However, it is unclear how it functions in eggs in which bicC mRNA is uniformly distributed, for instance, sea urchin eggs. Here, we show the function of BicC in the formation of neurogenic ectoderm of the sea urchin embryo. Loss-of-function experiments reveal that BicC is required for serotonergic neurogenesis and for expression of ankAT-1 gene, which is essential for the formation of apical tuft cilia in the neurogenic ectoderm of the sea urchin embryo. In contrast, the expression of FoxQ2, the neurogenic ectoderm specification transcription factor, is invariant in BicC morphants. Because FoxQ2 is an upstream factor of serotonergic neurogenesis and ankAT-1 expression, these data indicate that BicC functions in regulating the events that are coordinated by FoxQ2 during sea urchin embryogenesis.
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