Nanoindentation Response of 3D Printed PEGDA Hydrogels in a Hydrated Environment.
Nanoindentation Response of 3D Printed PEGDA Hydrogels in a Hydrated Environment.
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DOI：
10.1021/acsapm.2c01700
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发表时间：
2023-02-10
影响因子：
5
通讯作者：
Aria, Adrianus Indrat
中科院分区：
文献类型：
--
作者：
Khalili, Mohammad Hakim;Williams, Craig J.;Micallef, Christian;Duarte-Martinez, Fabian;Afsar, Ashfaq;Zhang, Rujing;Wilson, Sandra;Dossi, Eleftheria;Impey, Susan A.;Goel, Saurav;Aria, Adrianus Indrat
水凝胶是组织工程和器官芯片装置中常用的材料。本研究分别采用本体聚合和逐层投影光刻工艺制备了单片和多层聚（乙二醇）二丙烯酸酯（PEGDA）水凝胶，并研究了其纳米力学性能。层数从1增加到8和进一步到60（或层厚度减小）分别导致弹性模量从5.53减小到1.69和进一步到0.67MPa。发现层数的减少在三维（3D）打印的PEGDA水凝胶中诱导较低的蠕变指数（CIT）。这种减少归因于介观缺陷，其表现为归因于未反应的预聚物的局部区域的多层水凝胶的界面处的空隙袋，导致所检查的样品中的缺陷密度的变化。通过较高剂量的紫外线（UV）暴露引入的交联度的增加导致较高的弹性模量。这意味着水凝胶的弹性模量和蠕变行为受层和界面的交联度和缺陷密度的支配和影响。这些发现可以指导最佳的制造途径，以获得3D打印PEGDA水凝胶中所需的纳米机械性能，这对活细胞和组织的性能至关重要，可以通过控制制造参数进行工程设计。
Hydrogels are commonly used materials in tissue engineering and organ-on-chip devices. This study investigated the nanomechanical properties of monolithic and multilayered poly(ethylene glycol) diacrylate (PEGDA) hydrogels manufactured using bulk polymerization and layer-by-layer projection lithography processes, respectively. An increase in the number of layers (or reduction in layer thickness) from 1 to 8 and further to 60 results in a reduction in the elastic modulus from 5.53 to 1.69 and further to 0.67 MPa, respectively. It was found that a decrease in the number of layers induces a lower creep index (CIT) in three-dimensional (3D) printed PEGDA hydrogels. This reduction is attributed to mesoscale imperfections that appear as pockets of voids at the interfaces of the multilayered hydrogels attributed to localized regions of unreacted prepolymers, resulting in variations in defect density in the samples examined. An increase in the degree of cross-linking introduced by a higher dosage of ultraviolet (UV) exposure leads to a higher elastic modulus. This implies that the elastic modulus and creep behavior of hydrogels are governed and influenced by the degree of cross-linking and defect density of the layers and interfaces. These findings can guide an optimal manufacturing pathway to obtain the desirable nanomechanical properties in 3D printed PEGDA hydrogels, critical for the performance of living cells and tissues, which can be engineered through control of the fabrication parameters.
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影响因子：
14
作者：
Lu, Dongyuan;Luo, Chunhua;Long, Mian
通讯作者：
Long, Mian
影响因子：
1.9
作者：
Engler, AJ;Richert, L;Discher, DE
通讯作者：
Discher, DE
影响因子：
9.4
作者：
do Rosario, Jefferson J.;Haentsch, Yen;Lilleodden, Erica T.
通讯作者：
Lilleodden, Erica T.
DOI：
10.1016/j.msea.2013.12.091
发表时间：
2014-03-12
影响因子：
6.4
作者：
Goel, Saurav;Joshi, Suhas S.;Agrawal, Anupam
通讯作者：
Agrawal, Anupam
DOI：
10.1083/jcb.200405004
发表时间：
2004-09-13
期刊：
The Journal of cell biology
影响因子：
--
作者：
Engler AJ;Griffin MA;Sen S;Bönnemann CG;Sweeney HL;Discher DE
通讯作者：
Discher DE