Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures.
Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures.
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DOI：
10.3389/fnins.2021.689769
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发表时间：
2021
影响因子：
4.3
通讯作者：
Banerjee J
中科院分区：
文献类型：
--
作者：
Dey S;Doddamani RS;Banerjee Dixit A;Tripathi M;Sharma MC;Chandra PS;Banerjee J
据报道，WHO II级胶质瘤的瘤周区域，如星形细胞瘤和少突胶质细胞瘤，表现出癫痫样活动。谷氨酸和GABA能机制的失衡是癫痫样活动产生的主要原因。在这里，我们比较了术中有(GS)和无(GN)癫痫史的胶质瘤患者术中瘤周标本中锥体神经元的电生理特性。采用组织学和免疫组织化学方法检测瘤周组织中增殖细胞的浸润情况。采用全细胞膜片钳技术对11例GS和15例GN患者瘤周标本中锥体神经元自发的谷氨酸和氨基丁酸能活动进行检测。瘤周组织的细胞构筑缺乏Ki67免疫阳性细胞。我们观察到GS患者瘤周标本的锥体神经元上有较高频率的自发谷氨酸和GABA能活动。我们的研究结果表明，尽管GS和GN患者的组织病理学特征相似，但在自发的兴奋性和抑制性突触神经传递方面，GS和GN患者瘤周标本中的锥体神经元存在差异。突触后电流的改变可能与GS患者的自发癫痫样活动有关。
The peritumoral regions of WHO grade II gliomas, like astrocytoma and oligodendroglioma, have been reported to show epileptiform activities. An imbalance of glutamatergic and GABAergic mechanisms is primarily responsible for the generation of epileptiform activities. Here we have compared the electrophysiological properties of pyramidal neurons in intraoperative peritumoral specimens obtained from glioma patients with (GS) and without (GN) a history of seizures at presentation. Histology and immunohistochemistry were performed to assess the infiltration of proliferating cells at the peritumoral tissues. Whole-cell patch clamp technique was performed to measure the spontaneous glutamatergic and GABAergic activity onto pyramidal neurons in the peritumoral samples of GS (n = 11) and GN (n = 15) patients. The cytoarchitecture of the peritumoral tissues was devoid of Ki67 immuno-positive cells. We observed a higher frequency of spontaneous glutamatergic and GABAergic activities onto pyramidal neurons of the peritumoral samples of GS patients. Our findings suggest that, in spite of similar histopathological features, the pyramidal neurons in the peritumoral samples of GS and GN patients showed differences in spontaneous excitatory and inhibitory synaptic neurotransmission. An alteration in postsynaptic currents may contribute to the spontaneous epileptiform activity in GS patients.
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影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.4
作者：
Chang, Won Seok;Kim, Bong Soo;Chang, Jin Woo
通讯作者：
Chang, Jin Woo
影响因子：
6.1
作者：
Schidlitzki, Alina;Bascunana, Pablo;Loescher, Wolfgang
通讯作者：
Loescher, Wolfgang
影响因子：
2.2
作者：
Pitkanen, A;Tuunanen, J;Halonen, T
通讯作者：
Halonen, T
影响因子：
4.6
作者：
Lin CH;Hsu SP;Cheng TC;Huang CW;Chiang YC;Hsiao IH;Lee MH;Shen ML;Wu DC;Zhou N
通讯作者：
Zhou N