Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies.
Insights into the venom composition of the ectoparasitoid wasp Nasonia vitripennis from bioinformatic and proteomic studies.
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DOI：
10.1111/j.1365-2583.2009.00914.x
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发表时间：
2010-02
影响因子：
2.6
通讯作者：
Devreese B
中科院分区：
文献类型：
--
作者：
de Graaf DC;Aerts M;Brunain M;Desjardins CA;Jacobs FJ;Werren JH;Devreese B
有了玻喙纳索尼亚的基因组序列，我们试图通过两种不同的方法来确定毒液中存在的蛋白质。首先，我们利用已知膜翅目昆虫毒液蛋白的氨基酸序列，通过生物信息学方法寻找毒液蛋白的转录本。其次，我们利用离线二维液相色谱基质辅助激光解吸/电离飞行时间（2D-LC-MALDI-TOF）质谱（MS）和二维液相色谱电喷雾电离方正变换离子回旋共振（2D-LC-ESI-FT-ICR）质谱装置，对从毒液储存库中提取的玻璃螯合眼镜蛇粗毒液进行了蛋白质组学分析。这种生物信息学和蛋白质组学研究的结合产生了非常丰富的已确定的毒液成分。此外，在鉴定出的79种蛋白质中，有一半尚未与昆虫毒液相关：16种蛋白质仅与来自其他组织或分泌物的已知蛋白质相似，另外23种与任何已知蛋白质都没有相似之处。丝氨酸蛋白酶及其抑制剂是最具代表性的。通过蛋白质组学方法还鉴定了15种非分泌蛋白，可能代表所谓的“毒液微量元素”。本研究有助于从分子水平上了解拟寄生蜂毒液的生物多样性。
With the Nasonia vitripennis genome sequences available, we attempted to determine the proteins present in venom by two different approaches. First, we searched for the transcripts of venom proteins by a bioinformatic approach using amino acid sequences of known hymenopteran venom proteins. Second, we performed proteomic analyses of crude N. vitripennis venom removed from the venom reservoir, implementing both an off-line two-dimensional liquid chromatography matrix-assisted laser desorption/ionization time-of-flight (2D-LC-MALDI-TOF) mass spectrometry (MS) and a two-dimensional liquid chromatography electrospray ionization Founer transform ion cyclotron resonance (2D-LC-ESI-FT-ICR) MS setup. This combination of bioinformatic and proteomic studies resulted in an extraordinary richness of identified venom constituents. Moreover, half of the 79 identified proteins were not yet associated with insect venoms: 16 proteins showed similarity only to known proteins from other tissues or secretions, and an additional 23 did not show similarity to any known protein. Serine proteases and their inhibitors were the most represented. Fifteen nonsecretory proteins were also identified by proteomic means and probably represent so-called ‘venom trace elements’. The present study contributes greatly to the understanding of the biological diversity of the venom of parasitoid wasps at the molecular level.
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影响因子：
4.1
作者：
Faudry, Eric;Santana, Jaime M.;Teixeira, Antonio R. L.
通讯作者：
Teixeira, Antonio R. L.
DOI：
10.1046/j.0014-2956.2001.02685.x
发表时间：
2002-01-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Gáspári, Z;Patthy, A;Perczel, A
通讯作者：
Perczel, A
影响因子：
3.8
作者：
Falabella, Patrizia;Riviello, Lea;Pennacchio, Francesco
通讯作者：
Pennacchio, Francesco
影响因子：
2.9
作者：
Gopaul, DN;Meyer, SL;Schramm, VL
通讯作者：
Schramm, VL
影响因子：
2.9
作者：
GEORGATSOS, JG;LASKOWSKI, M
通讯作者：
LASKOWSKI, M