Comparative susceptibility to Plasmodium falciparum of the molecular forms M and S of Anopheles gambiae and Anopheles arabiensis.
Comparative susceptibility to Plasmodium falciparum of the molecular forms M and S of Anopheles gambiae and Anopheles arabiensis.
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DOI：
10.1186/1475-2875-10-269
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发表时间：
2011-09-19
期刊：
影响因子：
3
通讯作者：
Trape JF
中科院分区：
文献类型：
--
作者：
Ndiath MO;Cohuet A;Gaye A;Konate L;Mazenot C;Faye O;Boudin C;Sokhna C;Trape JF
冈比亚按蚊复合体的不同分类群表现出表型差异，这可能影响它们对疟疾传播的贡献。更具体地说，由于寄生虫和媒介之间的共同进化，它们对感染的敏感性可能是不同的。本研究的目的是比较冈比亚按蚊和阿拉伯按蚊M型和S型对恶性疟原虫感染的易感性。冈比亚按蚊F3代。在塞内加尔收集的恶性疟原虫感染者使用直接膜喂养，从自愿患者身上采集含有恶性疟原虫配子体的血液样本。7天后用光镜观察卵囊的存在，14天后用酶联免疫吸附试验检测子孢子的存在。用聚合酶链式反应鉴定蚊子的种类和分子类型。S分子体卵囊率(79.07%)显著高于M型(57.81%，费氏确切检验p；0.001)和阿拉伯按蚊(55.38%，费氏确切检验p<；0.001)。平均值±S.E.M.AN组卵囊数较多。冈比亚的S形(1.72±0.26)比An的(1.72±0.26)高。冈比亚M型为0.0001.6 4±0.0 4，P<0.0 1；阿拉伯组(0.0001.5 8±0.0 4)对S组(P<0.0 5)。S分子体子孢子率(83.52%)高于M体子孢子率(50.98%)和阿拉伯按蚊子孢子率(50.85%)(P<0.001)。在相同的实验条件下，S的分子形态为An。冈比亚对恶性疟原虫的感染比其分子形式M更敏感。冈比亚亚纲和An.阿拉伯。
The different taxa belonging to Anopheles gambiae complex display phenotypic differences that may impact their contribution to malaria transmission. More specifically, their susceptibility to infection, resulting from a co-evolution between parasite and vector, might be different. The aim of this study was to compare the susceptibility of M and S molecular forms of Anopheles gambiae and Anopheles arabiensis to infection by Plasmodium falciparum. F3 progenies of Anopheles gambiae s.l. collected in Senegal were infected, using direct membrane feeding, with P. falciparum gametocyte-containing blood sampled on volunteer patients. The presence of oocysts was determined by light microscopy after 7 days, and the presence of sporozoite by ELISA after 14 days. Mosquito species and molecular forms were identified by PCR. The oocyst rate was significantly higher in the molecular S form (79.07%) than in the M form (57.81%, Fisher's exact test p < 0.001) and in Anopheles arabiensis (55.38%, Fisher's exact test vs. S group p < 0.001). Mean ± s.e.m. number of oocyst was greater in the An. gambiae S form (1.72 ± 0.26) than in the An. gambiae M form (0.64 ± 0.04, p < 0.0001) and in the An. arabiensis group (0.58 ± 0.04, vs. S group, p < 0.0001). Sporozoite rate was also higher in the molecular form S (83.52%) than in form M (50.98%, Fisher's exact test p < 0.001) and Anopheles arabiensis 50.85%, Fisher's exact test vs. S group p < 0.001). Infected in the same experimental conditions, the molecular form S of An. gambiae is more susceptible to infection by P. falciparum than the molecular form M of An. gambiae and An. arabiensis.
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