AtKinesin-13A is located on Golgi-associated vesicle and involved in vesicle formation/budding in Arabidopsis root-cap peripheral cells.
AtKinesin-13A is located on Golgi-associated vesicle and involved in vesicle formation/budding in Arabidopsis root-cap peripheral cells.
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AtKinesin-13A 位于高尔基体相关囊泡上，参与拟南芥根冠外周细胞中的囊泡形成/出芽
DOI：
10.1186/1471-2229-9-138
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发表时间：
2009-11-25
影响因子：
5.3
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Wei L;Zhang W;Liu Z;Li Y
AtKinesin-13A 是来自拟南芥 (Arabidopsis thaliana) 的内部运动驱动蛋白。先前的免疫荧光结果表明 AtKinesin-13A 定位于植物细胞中的高尔基体堆栈。然而，其在高尔基体中的精确定位和生物学功能尚不清楚。在本文中，免疫荧光标记和共聚焦显微镜观察表明AtKinesin-13A与拟南芥根尖细胞中的高尔基体栈共定位。免疫电子显微镜观察表明，AtKinesin-13A 主要位于拟南芥根冠细胞中的高尔基体相关囊泡上。通过 T-DNA 插入，AtKinesin-13A 基因 (NM-112536) 失活导致根冠周围细胞中高尔基体囊泡的大小和数量急剧减少。同时，与野生型的相应细胞相比，这些细胞呈空泡化。这些结果表明，AtKinesin-13A 装饰高尔基体相关囊泡，并可能参与调节拟南芥根冠周围细胞中高尔基体囊泡的形成。
AtKinesin-13A is an internal-motor kinesin from Arabidopsis (Arabidopsis thaliana). Previous immunofluorescent results showed that AtKinesin-13A localized to Golgi stacks in plant cells. However, its precise localization and biological function in Golgi apparatus is unclear. In this paper, immunofluorescent labeling and confocal microscopic observation revealed that AtKinesin-13A was co-localized with Golgi stacks in Arabidopsis root tip cells. Immuno-electron microscopic observations indicated that AtKinesin-13A is primarily localized on Golgi-associated vesicles in Arabidopsis root-cap cells. By T-DNA insertion, the inactivation of the AtKinesin-13A gene (NM-112536) resulted in a sharp decrease of size and number of Golgi vesicles in root-cap peripheral cells. At the same time, these cells were vacuolated in comparison to the corresponding cells of the wild type. These results suggest that AtKinesin-13A decorates Golgi-associated vesicles and may be involved in regulating the formation of Golgi vesicles in the root-cap peripheral cells in Arabidopsis.
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