Cell division control in Escherichia coli K-12: some properties of the ftsZ84 mutation and suppression of this mutation by the product of a newly identified gene
Cell division control in Escherichia coli K-12: some properties of the ftsZ84 mutation and suppression of this mutation by the product of a newly identified gene
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大肠杆菌 K-12 中的细胞分裂控制：ftsZ84 突变的一些特性以及新鉴定基因产物对该突变的抑制
DOI：
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发表时间：
1988
影响因子：
3.2
通讯作者：
G. R. Drapeau
中科院分区：
文献类型：
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作者：
P. Phoenix;G. R. Drapeau
Fts蛋白在大肠杆菌的细胞分裂控制中起重要作用。这些可能形成功能复合物的蛋白质由形成操纵子的基因编码。在这项研究中，我们检测了由低拷贝数或高拷贝数质粒携带的温度敏感突变ftsZ84的特性。具有ftsZ84突变的菌株AB1157细胞在30℃的无盐L琼脂上没有形成菌落。当这些突变细胞中存在含有ftsZ84突变的低拷贝数质粒时，在30℃的培养基上菌落形成得以恢复，这表明ftsZ84可能比野生型蛋白活性低，因此限制了其触发细胞分裂的能力。另一方面，当高拷贝数质粒pBR325携带ftsZ84突变体时，无论受体是ftsZ84突变体还是亲本细胞，在无盐L琼脂板上集落形成都被阻止，这表明ftsZ84在高水平上起分裂抑制剂的作用。在42℃下菌落形成也被阻止，这表明FtsZ84蛋白在非允许温度下并没有失活。讨论了FtsZ84是一种比野生型FtsZ更有效的分裂抑制剂的可能性。也有证据表明，mutT附近的一个基因编码的产物，在某些条件下，抑制ftsZ84突变。
The Fts proteins play an important role in the control of cell division in Escherichia coli. These proteins, which possibly form a functional complex, are encoded by genes that form an operon. In this study, we examined the properties of the temperature-sensitive mutation ftsZ84 harbored by low- or high-copy-number plasmids. Cells of strain AB1157, which had the ftsZ84 mutation, did not form colonies on salt-free L agar at 30 degrees C. When a low-copy-number plasmid containing the ftsZ84 mutation was present in these mutant cells, colony formation was restored on this medium at 30 degrees C, suggesting that FtsZ84 is probably less active than the wild-type protein and is therefore limiting in its capacity to trigger cell divisions. On the other hand, when the ftsZ84 mutation was harbored by the high-copy-number plasmid pBR325, colony formation was prevented on salt-free L agar plates whether the recipients were ftsZ84 mutant or parental cells, suggesting that, at high levels, FtsZ84 acts as a division inhibitor. The fact that colony formation was also prevented at 42 degrees C indicates that the FtsZ84 protein is not inactivated at the nonpermissive temperature. The possibility that FtsZ84 is a more efficient division inhibitor than the wild-type FtsZ is discussed. Evidence is also presented showing that a gene adjacent to mutT codes for a product that, under certain conditions, suppresses the ftsZ84 mutation.
细胞分裂基因 ftsQ、ftsA 和 ftsZ 的结构和表达。
影响因子：
5.6
作者：
Yi,QM;Rockenbach,S;WardJr,JE;Lutkenhaus,J
通讯作者：
Lutkenhaus,J