Sewage reflects the distribution of human faecal Lachnospiraceae.
Sewage reflects the distribution of human faecal Lachnospiraceae.
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DOI：
10.1111/1462-2920.12092
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发表时间：
2013-08
影响因子：
5.1
通讯作者：
Sogin ML
中科院分区：
文献类型：
--
作者：
McLellan SL;Newton RJ;Vandewalle JL;Shanks OC;Huse SM;Eren AM;Sogin ML
粪便污染含有丰富多样的来自动物和人类的细菌群落，其中许多细菌可以作为传统肠球菌和大肠杆菌粪便指标的替代品。我们使用 16S rRNA 基因的大规模并行测序 (MPS) 来表征分布在美国 12 个城市的废水处理厂 (WWTP) 进水污水中的微生物群落。我们检查了梭菌目成员，其中包括梭菌科、毛螺菌科和瘤胃球菌科，以了解它们作为污水指标的潜力。 Lachnospiraceae 是污水中最丰富的粪便细菌类群之一，48 个人类粪便样本中至少有 46 个存在几种 Lachnospiraceae 高丰度污水热标记。针对污水样品中的球状梭菌 (C. coccoides) 的克隆文库表明，Lachnospiraceae 注释的 V6 热标记包含先前报道的球状梭菌组。我们使用寡聚型分析了 Lachnospiraceae 中的 Blautia 属，发现由 24 个熵成分组成的寡聚型，显示了宿主特异性的模式。这些发现表明，基于 Blautia 的指标可能具有区分不同粪便污染源的能力。制定特定水源的替代指标将加强水质评估，从而改善生态系统健康并减少水传播疾病造成的人类健康风险。
Faecal pollution contains a rich and diverse community of bacteria derived from animals and humans, many of which might serve as alternatives to the traditional enterococci and Escherichia coli faecal indicators. We used massively parallel sequencing (MPS) of the 16S rRNA gene to characterize microbial communities from wastewater treatment plant (WWTP) influent sewage from 12 cities geographically distributed across the USA. We examined members of the Clostridiales, which included the families Clostridiaceae, Lachnospiraceae and Ruminococcaceae for their potential as sewage indicators. Lachnospiraceae was one of the most abundant groups of faecal bacteria in sewage, and several Lachnospiraceae high-abundance sewage pyrotags occurred in at least 46 of 48 human faecal samples. Clone libraries targeting Clostridium coccoides (C. coccoides) in sewage samples demonstrated that Lachnospiraceae-annotated V6 pyrotags encompassed the previously reported C. coccoides group. We used oligotyping to profile the genus Blautia within Lachnospiraceae and found oligotypes comprised of 24 entropy components that showed patterns of host specificity. These findings suggest that indicators based on Blautia might have the capacity to discriminate between different faecal pollution sources. Development of source-specific alternative indicators would enhance water quality assessments, which leads to improved ecosystem health and reduced human health risk due to waterborne disease.
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影响因子：
4.4
作者：
Bernhard, AE;Field, KG
通讯作者：
Field, KG
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.
影响因子：
4.4
作者：
Fogarty, LR;Voytek, MA
通讯作者：
Voytek, MA
DOI：
10.1099/00207713-44-4-812
发表时间：
1994-10-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
COLLINS, MD;LAWSON, PA;FARROW, JAE
通讯作者：
FARROW, JAE
影响因子：
12.8
作者：
Fremaux, B.;Gritzfeld, J.;Yost, C. K.
通讯作者：
Yost, C. K.