The Presence of Hemoglobin in Cervicovaginal Lavage Is Not Associated With Genital Schistosomiasis in Zambian Women From the BILHIV Study.
The Presence of Hemoglobin in Cervicovaginal Lavage Is Not Associated With Genital Schistosomiasis in Zambian Women From the BILHIV Study.
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DOI：
10.1093/ofid/ofac586
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发表时间：
2022-12
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
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作者：
女性生殖器血吸虫病(FGS)是血吸虫虫卵沉积在生殖组织中时发生的。宫颈粘膜中的女性生殖器血吸虫病与血管增多有关。如果FGS与宫颈阴道灌洗液(CVL)中血红蛋白的存在有关，那么使用尿试纸条检测CVL中的血红蛋白可以补充FGS的诊断。参加HPTN 071(PopART)人群队列的18-31岁未经、未怀孕、性活跃的妇女被邀请到赞比亚的两个社区。在家庭访问中收集生殖器自身拭子和尿样，在助产士引导的诊所访问中进行CVL和手持阴道镜检查。用尿试纸条鉴定CVL患者的血红蛋白。尿检虫卵和循环阳极抗原(CAA)。VISUAL-FGS的定义为存在沙斑、橡胶丘疹或异常血管。聚合酶链式反应(PCR)-FGS被定义为在CVL或宫颈或阴道拭子上实时PCR检测到的血吸虫脱氧核糖核酸。在209名有家庭生殖器拭子和伴发CVL样本的妇女中，66%(138/209)有可检测到的CVL血红蛋白，13.4%(28/209)有PCR定义的FGS，17.2%(36/209)有视觉FGS。经CAA或尿液镜检诊断为活动性血吸虫感染的参与者中有21.0%(44/209)。活动性血吸虫感染(P=0.4)、PCR-FGS(P=0.7)和视觉FGS(P=0.3)与CVL血红蛋白的存在无关。结果在感染负荷高的亚组中没有不同(周期阈值<；35或2-3个生殖器PCR阳性)。聚合酶链式反应-FGS、视觉-FGS和血吸虫活动性感染与CVL血红蛋白的存在无关。需要进一步研究，以建立可利用的、基于社区的FGS诊断方法。尿试纸条可用于估计S血吸虫感染情况。女性生殖器血吸虫病是S血吸虫感染的并发症，与宫颈粘膜病变有关。本研究评价URS宫颈阴道灌洗对FGS的诊断价值。
Female genital schistosomiasis (FGS) occurs when Schistosoma haematobium eggs are deposited in reproductive tissue. Female genital schistosomiasis in the cervical mucosa is associated with increased vascularity. If FGS is associated with the presence of hemoglobin in cervicovaginal lavage (CVL), the use of urinary reagent strips to detect hemoglobin in CVL could supplement FGS diagnosis. Nonmenstruating, nonpregnant, sexually active women aged 18–31 participating in the HPTN 071 (PopART) Population-Cohort were invited in 2 Zambian communities. Genital self-swabs and a urine specimen were collected at a home visit, and CVL and hand-held colposcopy were performed at a midwife led clinic visit. Urinary reagent strips were used to identify hemoglobin in CVL. Eggs and circulating anodic antigen (CAA) were detected from urine. Visual-FGS was defined as the presence of sandy patches, rubbery papules, or abnormal blood vessels. Polymerase chain reaction (PCR)-FGS was defined as Schistosoma deoxyribonucleic acid detected by real-time PCR on CVL or cervical or vaginal swab. Of 209 women with home genital swabs and companion CVL specimens, 66% (138 of 209) had detectable CVL hemoglobin, 13.4% (28 of 209) had PCR-defined FGS, and 17.2% (36 of 209) had visual-FGS. Active Schistosoma infection, diagnosed by CAA or urine microscopy, was present in 21.0% (44 of 209) participants. Active Schistosoma infection (P = .4), PCR-FGS (P = 0.7), and visual-FGS (P = 0.3) were not associated with CVL hemoglobin presence. Results did not differ in subgroups with high infection burden (cycle threshold < 35 or 2–3 positive genital PCR). Polymerase chain reaction-FGS, visual-FGS, and active Schistosoma infection were not associated with the presence of CVL hemoglobin. Further research is needed to establish accessible community-based FGS diagnostics. Urinary reagent strips (URS) are useful for estimating S haematobium prevalence. Female genital schistosomiasis (FGS) occurs as a complication of S haematobium infection and is associated with cervical mucosal changes. This study evaluates URS in cervicovaginal lavage for FGS diagnosis.
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影响因子：
3.2
作者：
Krauth SJ;Greter H;Stete K;Coulibaly JT;Traoré SI;Ngandolo BN;Achi LY;Zinsstag J;N'Goran EK;Utzinger J
通讯作者：
Utzinger J
影响因子：
3.7
作者：
Francis SC;Hou Y;Baisley K;van de Wijgert J;Watson-Jones D;Ao TT;Herrera C;Maganja K;Andreasen A;Kapiga S;Coulton GR;Hayes RJ;Shattock RJ
通讯作者：
Shattock RJ
影响因子：
3.8
作者：
通讯作者：
--
影响因子：
8.4
作者：
Ochodo, Eleanor A.;Gopalakrishna, Gowri;Spek, Bea;Reitsma, Johannes B.;van Lieshout, Lisette;Polman, Katja;Lamberton, Poppy;Bossuyt, Patrick M. M.;Leeflang, Mariska M. G.
通讯作者：
Leeflang, Mariska M. G.
影响因子：
3.8
作者：
Knopp, Stefanie;Corstjens, Paul L. A. M.;van Dam, Govert J.
通讯作者：
van Dam, Govert J.