Characteristics of hydrogen evolution and oxidation catalyzed by Desulfovibrio caledoniensis biofilm on pyrolytic graphite electrode
Characteristics of hydrogen evolution and oxidation catalyzed by Desulfovibrio caledoniensis biofilm on pyrolytic graphite electrode
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喀里多尼亚脱硫弧菌生物膜在热解石墨电极上催化析氢和氧化的特性
DOI：
10.1016/j.electacta.2011.05.086
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发表时间：
2011-10
影响因子：
6.6
通讯作者：
Baorong Hou
中科院分区：
文献类型：
--
作者：
(3) Lin Yu;Jizhou Duan;Wei Zhao;Yanliang Huang;Baorong Hou
氢化酶是硫酸盐还原细菌（SRB）中一种重要的电活性酶，它通过催化氢的析出和氧化而将其活性与固体电极连接起来。然而，SRB的类似电活性特性却很少受到关注。研究了SRB生物膜包覆的热解石墨电极的电活性。循环伏安法检测到两个对应的氧化还原峰，这两个峰与析氢和氧化有关。此外，SRB生物膜的存在降低了反应的过电位约0.2V。当SRB生物膜包裹的PGE在0.24V （vs. SHE）下极化时，发现了与氢氧化相关的氧化电流。通过无损电化学阻抗谱检测，SRB生物膜能够从−0.61V （vs. SHE）极化的PGE中获得电子形成氢气，并且电子传递电阻也随着SRB生物膜的形成而减小。结果表明，厌氧环境下，析氢氧化是SRB生物膜与固体电极之间电子传递的重要途径。
Hydrogenase, an important electroactive enzyme of sulphate-reducing bacteria (SRB), has been discovered having the capacity to connect its activity to solid electrodes by catalyzing hydrogen evolution and oxidation. However, little attention has been paid to similar electroactive characteristics of SRB. In this study, the electroactivities of pyrolytic graphite electrode (PGE) coated with SRB biofilm were investigated. Two corresponding redox peaks were observed by cyclic voltammetry detection, which were related to the hydrogen evolution and oxidation. Moreover, the overpotential for the reactions decreased by about 0.2V in the presence of the SRB biofilm. When the PGE coated with the SRB biofilm was polarized at 0.24V (vs. SHE), an oxidation current related to the hydrogen oxidation was found. The SRB biofilm was able to obtain electrons from the −0.61V (vs. SHE) polarized PGE to form hydrogen, and the electron transfer resistance also decreased with the formation of SRB biofilm, as measured by the non-destructive electrochemical impendence spectroscopy detection. It was concluded that the hydrogen evolution and oxidation was an important way for the electron transfer between SRB biofilm and solid electrode in anaerobic environment.
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影响因子：
16.1
作者：
B. Little;P. Wagner;F. Mansfeld
通讯作者：
B. Little;P. Wagner;F. Mansfeld
影响因子：
--
作者：
J. Crolet;M. Magot
通讯作者：
J. Crolet;M. Magot
影响因子：
15
作者：
Rüdiger, O;Abad, JM;De Lacey, AL
通讯作者：
De Lacey, AL
影响因子：
4.5
作者：
LAVIRON, E
通讯作者：
LAVIRON, E
影响因子：
11.4
作者：
Geelhoed, Jeanine S.;Stams, Alfons J. M.
通讯作者：
Stams, Alfons J. M.