Effects of scutellarin on MUC5AC mucin production induced by human neutrophil elastase or interleukin 13 on airway epithelial cells.
Effects of scutellarin on MUC5AC mucin production induced by human neutrophil elastase or interleukin 13 on airway epithelial cells.
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灯盏乙素对人中性粒细胞弹性蛋白酶或白细胞介素 13 诱导气道上皮细胞 MUC5AC 粘蛋白产生的影响
DOI：
10.3346/jkms.2011.26.6.778
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发表时间：
2011-06
影响因子：
4.5
通讯作者：
Zhou XD
中科院分区：
文献类型：
--
作者：
Jiang DP;Perelman JM;Kolosov VP;Zhou XD
灯盏乙素是从传统中药灯盏花中提取的一种黄酮类化合物。本研究探讨了灯盏乙素对MUC 5AC细胞分泌粘蛋白的影响及其可能的机制。用灯盏乙素预处理人支气管上皮16（HBE 16）细胞60 min，再用中性粒细胞弹性蛋白酶（HNE）或白细胞介素（IL）-13作用12 h，用RT-PCR和ELISA检测MUC 5AC粘蛋白的产生量。结果表明，灯盏乙素在mRNA和蛋白水平上均抑制HNE诱导的MUC 5AC表达，并呈浓度依赖性。然而，灯盏乙素未能抑制IL-13诱导的MUC 5AC粘蛋白产生。为探讨灯盏乙素影响MUC 5AC粘蛋白产生的细胞内机制，采用蛋白质印迹法检测蛋白激酶C（PKC）、信号转导和转录激活因子6（STAT 6）和细胞外信号调节激酶1/2（ERK 1/2）的磷酸化水平。野黄芩苷处理后，PKC和ERK 1/2的磷酸化作用减弱，而STAT 6则没有受到显着影响。因此，表明灯盏乙素通过ERK依赖性和PKC依赖性途径下调HBE 16细胞上MUC 5AC粘蛋白的产生。
Scutellarin is a flavonoid extracted from a traditional Chinese herb, Erigeron breviscapus. The present study investigated the effect of scutellarin on MUC5AC mucin production and the possible mechanism. Human bronchial epithelial 16 (HBE16) cells were pretreated with scutellarin for 60 min, and then exposed to human neutrophil elastase (HNE) or interleukin (IL)-13 for 12 hr. RT-PCR and ELISA were performed to measure the amount of MUC5AC mucin production. The results showed that scutellarin inhibited MUC5AC expression both in mRNA and protein level induced by HNE in a concentration-dependent manner. However, scutellarin failed to inhibit MUC5AC mucin production induced by IL-13. To investigate the intracellular mechanisms associated with the effect of scutellarin on MUC5AC mucin production, western blotting was carried out to examine the phosphorylation of protein kinase C (PKC), signal transducer and activator of transcription 6 (STAT6) and extracellular signal-regulated kinase 1/2 (ERK1/2). The phosphorylation of PKC and ERK1/2 was attenuated after treatment with scutellarin, whereas STAT6 was not significantly affected. Therefore, it is suggested that scutellarin down-regulates MUC5AC mucin production on HBE16 cells via ERK-dependent and PKC-dependent pathways.
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DOI：
10.1152/ajplung.00140.2004
发表时间：
2004-12-01
影响因子：
4.9
作者：
Voynow, JA;Fischer, BM;Foster, WM
通讯作者：
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DOI：
10.1124/jpet.110.171561
发表时间：
2011-01-01
影响因子：
3.5
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影响因子：
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影响因子：
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Hong, H;Liu, GQ
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