The next-generation nicotine vaccine: a novel and potent hybrid nanoparticle-based nicotine vaccine.
The next-generation nicotine vaccine: a novel and potent hybrid nanoparticle-based nicotine vaccine.
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DOI：
10.1016/j.biomaterials.2016.08.028
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发表时间：
2016-11
期刊：
影响因子：
14
通讯作者：
Zhang, Chenming
中科院分区：
文献类型：
--
作者：
Hu, Yun;Smith, Daniel;Frazier, Evan;Hoerle, Reece;Ehrich, Marion;Zhang, Chenming
由于迫切需要更有效的治疗尼古丁成瘾，本研究开发了一种基于纳米颗粒的混合尼古丁疫苗（NanoNiccine）。NanoNiccine由聚（丙交酯-共-乙交酯）酸（PLGA）核心、包封在PLGA核心内的作为佐剂蛋白的钥孔血蓝蛋白（KLH）、脂质层和与脂质层外表面偶联的尼古丁半抗原组成。与传统的尼古丁疫苗相比，NanoNiccine不是尼古丁-蛋白质结合疫苗。相反，尼古丁半抗原和蛋白质分别位于纳米结构中，以最小化针对KLH的抗体产生。细胞摄取研究表明，NanoNiccine对于树突状细胞（DC）的内化和加工是理想的。与用传统尼古丁-KLH（Nic-KLH）疫苗免疫的小鼠相比，用NanoNiccine免疫的小鼠产生针对KLH的低得多的IgG水平。此外，NanoNiccine实现的抗尼古丁IgG滴度比阳性对照Nic-KLH高400%。此外，NanoNiccine的Th 1/Th 2指数表明，NanoNiccine诱导的免疫应答为抗体应答主导。此外，发现NanoNiccine在小鼠中是安全的。
Owing to the urgent need for more effective treatment against nicotine addiction, a hybrid nanoparticle-based nicotine vaccine (NanoNiccine) was developed in this study. NanoNiccine was composed of a poly(lactide-co-glycolide) acid (PLGA) core, keyhole limpet hemocyanin (KLH) as an adjuvant protein enclosed within the PLGA core, a lipid layer, and nicotine haptens conjugated to the outer surface of the lipid layer. In contrast to the traditional nicotine vaccine, NanoNiccine is not a nicotine-protein conjugate vaccine. Instead, the nicotine hapten and protein are separately located in the nanostructure to minimize antibody production towards KLH. The cellular uptake study demonstrated that NanoNiccine was ideal for internalization and processing by dendritic cells (DCs). Mice immunized with NanoNiccine produced much lower IgG level against KLH as compared to that immunized with the traditional nicotine-KLH (Nic-KLH) vaccine. In addition, NanoNiccine achieved up to a 400% higher titer of anti-nicotine IgG than the positive control, Nic-KLH. Additionally, the Th1/Th2 index of NanoNiccine suggested that the immune response induced by NanoNiccine was antibody response dominant. Furthermore, NanoNiccine was found to be safe in mice.
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影响因子：
9.7
作者：
Hu, Yun;Hoerle, Reece;Zhang, Chenming
通讯作者：
Zhang, Chenming
影响因子：
14
作者：
Cheng, Jianjun;Teply, Benjamin A.;Farokhzad, Omid C.
通讯作者：
Farokhzad, Omid C.
影响因子：
14
作者：
Dong, YC;Feng, SS
通讯作者：
Feng, SS
影响因子：
168.9
作者：
Giovino, Gary A.;Mirza, Sara A.;Asma, Samira
通讯作者：
Asma, Samira
影响因子：
5.5
作者：
HogenEsch, H
通讯作者：
HogenEsch, H