Isolation and characterization of a tonoplast Na+/H+ antiporter from the halophyte Nitraria sibirica
Isolation and characterization of a tonoplast Na+/H+ antiporter from the halophyte Nitraria sibirica
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盐生植物西伯利亚白刺 (Nitraria sibirica) 液泡膜 Na /H 逆向转运蛋白的分离和表征
DOI：
10.1007/s10535-015-0560-8
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发表时间：
2015
影响因子：
1.5
通讯作者：
Lin Xiaofei
中科院分区：
文献类型：
--
作者：
Li Ningning;Zhang Wenbo;Mao Huiping;Lin Xiaofei
Na+/H+交换器(NHx)介导的Na+和H+逆向转运是植物耐盐性的重要机制。本研究从盐生植物西伯利亚白刺中分离到安娜+/H+逆向转运蛋白基因NsNHX1。采用简并聚合酶链式反应(PCR)和快速扩增c DNA末端(RACE)技术。NsNHX1基因全长2 182个碱基，包含一个1 635个碱基的开放阅读框(ORF)，编码544个氨基酸，与其他植物的液泡膜定位的NHXs序列具有显著的相似性。亚细胞定位分析证实NsNHX1为液泡膜定位蛋白。NsNHX1启动子区域存在与生物和非生物胁迫相关的顺式元件，逆转录-聚合酶链式反应证实NsNHX1的表达受外源脱落酸(ABA)、低温和氯化钠的诱导。200 mM盐处理3h后，NsNHX1的转录急剧增加，表明NsNHX1对盐胁迫反应迅速。NsNHX1基因的过表达提高了转基因拟南芥的耐盐性，表明NsNHX1介导的Na+区域化在提高植物耐盐性方面起着重要作用。
Na+/H+ exchanger (NHX)-mediated Na+ and H+ antiport is an important mechanism for salt tolerance in plants. In this study, an Na+/H+ antiporter gene, referred to as NsNHX1, was isolated from the halophyte Nitraria sibirica Pall. using degenerate polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE). The resulting 2 182 bp NsNHX1 cDNA contained a 1 635 bp open reading frame (ORF) that encoded 544 amino acids and showed striking sequence similarity to tonoplast-localized NHXs from other plants. Subcellular localization analysis confirmed NsNHX1 to be a tonoplast-localized protein. Cis-elements described as being responsive to biotic and abiotic stresses were present in the NsNHX1 promoter region, and reverse transcription (RT)-PCR analysis confirmed that NsNHX1 expression was induced by exogenous abscisic acid (ABA), cold, and NaCl. Transcription of NsNHX1 increased sharply 3 h after treatment with 200 mM NaCl revealing that NsNHX1 responded rapidly to the salt stress. Overexpression of NsNHX1 enhanced salt tolerance in transgenic Arabidopsis thalliana L. suggesting that NsNHX1-mediated Na+ compartmentalization played an important role in enhancing plant salt tolerance.
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影响因子：
5.6
作者：
Chanroj S;Wang G;Venema K;Zhang MW;Delwiche CF;Sze H
通讯作者：
Sze H
DOI：
10.1007/978-1-61779-986-0
发表时间：
2012
期刊：
--
影响因子：
--
作者：
S. Shabala;T. Cuin
通讯作者：
S. Shabala;T. Cuin
DOI：
10.1007/978-3-540-39402-0_10
发表时间：
2004-07
期刊：
--
影响因子：
--
作者：
V. Chinnusamy;Jian-Kang Zhu
通讯作者：
V. Chinnusamy;Jian-Kang Zhu
影响因子：
64.8
作者：
Fukada-Tanaka, S;Inagaki, Y;Iida, S
通讯作者：
Iida, S
影响因子：
4.3
作者：
Guo-Qiang Wu;Jiejun Xi;Qian Wang;Aike Bao;Q. Ma;Jinlin Zhang;Suo-min Wang
通讯作者：
Guo-Qiang Wu;Jiejun Xi;Qian Wang;Aike Bao;Q. Ma;Jinlin Zhang;Suo-min Wang