Metabolically Engineered Escherichia coli for Conversion of D-Fructose to D-Allulose via Phosphorylation-Dephosphorylation.
Metabolically Engineered Escherichia coli for Conversion of D-Fructose to D-Allulose via Phosphorylation-Dephosphorylation.
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代谢设计的大肠杆菌通过磷酸化 - 脱磷酸化将D-果糖转化为D-果糖。
DOI：
10.3389/fbioe.2022.947469
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发表时间：
2022
影响因子：
5.7
通讯作者：
Lin, Xiao-Cheng
中科院分区：
文献类型：
--
作者：
Guo, Qiang;Liu, Chen-Yang;Zheng, Ling-Jie;Zheng, Shang-He;Zhang, Ya-Xing;Zhao, Su-Ying;Zheng, Hui-Dong;Fan, Li-Hai;Lin, Xiao-Cheng
D-Allulose is an ultra-low calorie sweetener with broad market prospects. As an alternative to Izumoring, phosphorylation-dephosphorylation is a promising method for D-allulose synthesis due to its high conversion of substrate, which has been preliminarily attempted in enzymatic systems. However, in vitro phosphorylation-dephosphorylation requires polyphosphate as a phosphate donor and cannot completely deplete the substrate, which may limit its application in industry. Here, we designed and constructed a metabolic pathway in Escherichia coli for producing D-allulose from D-fructose via in vivo phosphorylation-dephosphorylation. PtsG-F and Mak were used to replace the fructose phosphotransferase systems (PTS) for uptake and phosphorylation of D-fructose to fructose-6-phosphate, which was then converted to D-allulose by AlsE and A6PP. The D-allulose titer reached 0.35 g/L and the yield was 0.16 g/g. Further block of the carbon flux into the Embden-Meyerhof-Parnas (EMP) pathway and introduction of an ATP regeneration system obviously improved fermentation performance, increasing the titer and yield of D-allulose to 1.23 g/L and 0.68 g/g, respectively. The E. coli cell factory cultured in M9 medium with glycerol as a carbon source achieved a D-allulose titer of ≈1.59 g/L and a yield of ≈0.72 g/g on D-fructose.
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影响因子：
16.6
作者：
Dugar G;Svensson SL;Bischler T;Wäldchen S;Reinhardt R;Sauer M;Sharma CM
通讯作者：
Sharma CM
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
--
影响因子：
6.4
作者：
Su L;Sun F;Liu Z;Zhang K;Wu J
通讯作者：
Wu J
DOI：
10.1073/pnas.120163297
发表时间：
2000-06-06
影响因子：
11.1
作者：
Datsenko, KA;Wanner, BL
通讯作者：
Wanner, BL
解纤维梭菌 H10 的 d-阿洛酮糖 3-差向异构酶的克隆、表达和表征
影响因子：
6.1
作者：
Mu, Wanmeng;Chu, Feifei;Jiang, Bo
通讯作者：
Jiang, Bo