Passive and active shaping of unitary responses from associational/commissural and perforant path synapses in hippocampal CA3 pyramidal cells.
Passive and active shaping of unitary responses from associational/commissural and perforant path synapses in hippocampal CA3 pyramidal cells.
复制标题
DOI：
10.1007/s10827-010-0303-y
复制
发表时间：
2011-10
影响因子：
1.2
通讯作者：
Barrionuevo, German
中科院分区：
文献类型：
--
作者：
Perez-Rosello, Tamara;Baker, John L.;Ferrante, Michele;Iyengar, Satish;Ascoli, Giorgio A.;Barrionuevo, German
虽然对CA 3b锥体细胞的联合/连合（A/C）和穿通通路（PP）输入在海马记忆功能中起着中心作用，但形成A/C和PP的主动和被动过程AMPA和NMDA受体介导的单一EPSP/EPSC（AMPA和NMDA uEPSP/uEPSC）尚未完全表征。在这里，我们发现无论是AMPA或NMDA uEPSPs的A/C和PP之间的躯体振幅没有差异。然而，从近端比从远端A/C或PP诱发更大的AMPA uEPSC。考虑到CA 3锥体细胞中的空间钳限制，这些电压钳数据表明A/C和PP AMPA uEPSP振幅的位置无关性部分地通过激活索马处或附近的电压依赖性电导来实现。此外，远端A/C和PP的uEPSC振幅的相似性表明了非箝位有源电导的额外参与。事实上，药理学阻断电压依赖性电导消除了这些输入的位置无关性。与此相反，在所有条件下，A/C和PP NMDA uEPSP/uEPSC振幅的位置无关性保持，表明传播不受活性膜过程的影响。A/C uEPSP振幅的位置无关性可能与其他CA 3锥体细胞募集CA 3锥体细胞有关。这些数据还表明，PP兴奋代表了一个重要的输入CA 3锥体细胞。被动数据对局部突触特性的影响将在配套文件中进一步研究，并提供详细的计算模型。
Although associational/commissural (A/C) and perforant path (PP) inputs to CA3b pyramidal cells play a central role in hippocampal mnemonic functions, the active and passive processes that shape A/C and PP AMPA and NMDA receptor-mediated unitary EPSP/EPSC (AMPA and NMDA uEPSP/uEPSC) have not been fully characterized yet. Here we find no differences in somatic amplitude between A/C and PP for either AMPA or NMDA uEPSPs. However, larger AMPA uEPSCs were evoked from proximal than from distal A/C or PP. Given the space-clamp constraints in CA3 pyramidal cells, these voltage clamp data suggest that the location-independence of A/C and PP AMPA uEPSP amplitudes is achieved in part through the activation of voltage dependent conductances at or near the soma. Moreover, similarity in uEPSC amplitudes for distal A/C and PP points to the additional participation of unclamped active conductances. Indeed, the pharmacological blockade of voltage-dependent conductances eliminates the location-independence of these inputs. In contrast, the location-independence of A/C and PP NMDA uEPSP/uEPSC amplitudes is maintained across all conditions indicating that propagation is not affected by active membrane processes. The location-independence for A/C uEPSP amplitudes may be relevant in the recruitment of CA3 pyramidal cells by other CA3 pyramidal cells. These data also suggest that PP excitation represents a significant input to CA3 pyramidal cells. Implication of the passive data on local synaptic properties is further investigated in the companion paper with a detailed computational model.
登录
查看更多内容
影响因子：
2.5
作者：
BLACKSTAD, TW;BRINK, K;JEUNE, B
通讯作者：
JEUNE, B
影响因子：
2.5
作者：
Fernandez de Sevilla, David;Fuenzalida, Marco;Buno, Washington
通讯作者：
Buno, Washington
DOI：
10.1016/j.molbrainres.2004.12.005
发表时间：
2005-04-27
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Coultrap, SJ;Nixon, KM;Browning, MD
通讯作者：
Browning, MD
影响因子：
2.5
作者：
Araki, I;deGroat, WC
通讯作者：
deGroat, WC
影响因子：
5.5
作者：
Chitwood, RA;Hubbard, A;Jaffe, DB
通讯作者：
Jaffe, DB