EVIDENCE FOR INTROGRESSIVE HYBRIDIZATION BASED ON CHLOROPLAST DNA POLYMORPHISMS AND MORPHOLOGICAL VARIATION IN WILD EVERGREEN AZALEA POPULATIONS OF THE KIRISHIMA MOUNTAINS, JAPAN
EVIDENCE FOR INTROGRESSIVE HYBRIDIZATION BASED ON CHLOROPLAST DNA POLYMORPHISMS AND MORPHOLOGICAL VARIATION IN WILD EVERGREEN AZALEA POPULATIONS OF THE KIRISHIMA MOUNTAINS, JAPAN
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基于日本雾岛山野生常绿杜鹃花种群叶绿体 DNA 多态性和形态变异的渐渗杂交证据
DOI：
10.1017/s0960428600000147
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发表时间：
2000
影响因子：
--
通讯作者：
K. Takayanagi
中科院分区：
文献类型：
--
作者：
N. Kobayashi;T. Handa;K. Yoshimura;Y. Tsumura;K. Arisumi;K. Takayanagi
为了确定园艺万年青杜鹃花品种的起源，本研究集中在九州（日本南部主岛）杜鹃花的自然种群。雾岛山脉是九州的火山山脉，是日本万年青杜鹃花的重要多样性中心。高山杜鹃生长在海拔1000米以上，而R.山柰分布在海拔600米以下。这两个物种的自然杂交群体被发现在中间区域（海拔1000- 600米）。这两个物种已明确区分其各自的形态特征。高山杜鹃花粉红紫色，叶小椭圆形;山柰有较大的橙红色的花，带有深色斑点和大的长方形叶子。种间杂种表现出两个物种范围内的表型，特别是关于花色和叶形。在这两个种之间观察到了这些特征的形态学渐变。叶绿体DNA的PCR-RFLP分析在16 S rDNA区域检测到两种植物的特异性条带。种间杂种群体由具有R. kiusianum或R.山柰。这表明R. kiusianum和R.山柰是明显不同的物种。此外，天然杂种群体由具有两个cpDNA之一的个体组成。在R. kiusianum（T-1430和T-1030）具有R.这表明，在R. kaempferi种群中已经发生了细胞质渐渗。kiusianum从R.山柰。
For the purpose of determining the origin of horticultural evergreen azalea cultivars, this study was focused on the natural populations of azalea in Kyushu (south main island of Japan). The Kirishima mountains, the volcanic mountain mass in Kyushu, are an important centre of diversity for the Japanese evergreen azaleas. Rhododendron kiusianum Makino grows above 1000m alt., whereas R. kaempferi Planch. is distributed below 600m alt. Putative natural hybrid populations of these two species are found in the intermediate region (1000–600m alt.). These two species have been clearly distinguished by their respective morphological features. Rhododendron kiusianum has small pink-purple flowers and small elliptical leaves, whereas R. kaempferi has larger red-orange flowers with dark blotches and large oblong leaves. Interspecific hybrids show phenotypes within the range of the two species, especially with regard to flower colour and leaf shape. A morphological cline of these characteristics corresponding to altitude has been observed between these two species. PCR-RFLP analysis of chloroplast DNA detected specific bands for the two species in the 16S rDNA region when digested with Hha I restriction enzyme. Populations of interspecific hybrids were composed of individuals that had a banding pattern of either R. kiusianum or R. kaempferi . This indicates that R. kiusianum and R. kaempferi are clearly distinct species. Furthermore, natural hybrid populations consist of individuals that have one of two cpDNA. Some individuals in the populations of R. kiusianum (T-1430 and T-1030) possess the cpDNA pattern of R. kaempferi , which suggests that cytoplasmic introgression has occurred in the populations of R. kiusianum from R. kaempferi .
影响因子：
11.4
作者：
SHINOZAKI, K;OHME, M;SUGIURA, M
通讯作者：
SUGIURA, M