The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.
The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export.
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DOI：
10.1091/mbc.e09-12-1041
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发表时间：
2010-06-01
影响因子：
3.3
通讯作者：
Yoneda Y
中科院分区：
文献类型：
--
作者：
Oka M;Asally M;Yasuda Y;Ogawa Y;Tachibana T;Yoneda Y
Nup98是一种可移动的核孔蛋白，在细胞核中形成不同的点。我们的研究结果表明，Nup98在crm1介导的核输出中起着新的穿梭辅因子的作用。Nup98是一种可移动的核孔蛋白，在细胞核中形成不同的点，尽管Nup98在核运输中的作用已被提出，但其确切功能尚不清楚。在这里，我们发现Nup98在crm1介导的核蛋白输出中起重要作用。用leptomycin B（一种核输出受体Crm1的特异性抑制剂）处理后，细胞核而非细胞质中egfp标记的Nup98点迅速消失。突变分析表明，Nup98在物理和功能上通过其n端苯丙氨酸-甘氨酸（FG）重复区以依赖于rangtp的方式与Crm1相互作用。此外，Nup98-Crm1复合物的活性由RanBP3调节，RanBP3是已知的crm1介导的核输出的辅助因子。最后，胞质微注射抗nup98抑制了crm1依赖的细胞核蛋白输出，同时在细胞核中积累了抗nup98。这些结果清楚地表明，Nup98与RanBP3一起作为crm1介导的核输出的新型穿梭辅因子发挥作用。
Nup98 is a mobile nucleoporin that forms distinct dots in the nucleus. Our results show that Nup98 functions as a novel shuttling cofactor in Crm1-mediated nuclear export. Nup98 is a mobile nucleoporin that forms distinct dots in the nucleus, and, although a role for Nup98 in nuclear transport has been suggested, its precise function remains unclear. Here, we show that Nup98 plays an important role in Crm1-mediated nuclear protein export. Nuclear, but not cytoplasmic, dots of EGFP-tagged Nup98 disappeared rapidly after cell treatment with leptomycin B, a specific inhibitor of the nuclear export receptor, Crm1. Mutational analysis demonstrated that Nup98 physically and functionally interacts with Crm1 in a RanGTP-dependent manner through its N-terminal phenylalanine-glycine (FG) repeat region. Moreover, the activity of the Nup98-Crm1 complex was modulated by RanBP3, a known cofactor for Crm1-mediated nuclear export. Finally, cytoplasmic microinjection of anti-Nup98 inhibited the Crm1-dependent nuclear export of proteins, concomitant with the accumulation of anti-Nup98 in the nucleus. These results clearly demonstrate that Nup98 functions as a novel shuttling cofactor for Crm1-mediated nuclear export in conjunction with RanBP3.
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影响因子：
64.5
作者：
Fornerod, M;Ohno, M;Mattaj, IW
通讯作者：
Mattaj, IW
RanBP3 独立于 CRM1 增强活性 (β)-连环蛋白的核输出。
影响因子：
7.8
作者：
Hendriksen, J;Fagotto, F;van der Velde, H;van Schie, M;Noordermeer, J;Fornerod, M
通讯作者：
Fornerod, M
影响因子：
3.3
作者：
Griffis, ER;Altan, N;Powers, MA
通讯作者：
Powers, MA
影响因子：
3.9
作者：
Dasso, M.
通讯作者：
Dasso, M.
影响因子：
7.8
作者：
Bastos, R;dePouplana, LR;Burke, B
通讯作者：
Burke, B