Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts

Analysis of Ascorbic Acid Biosynthesis Using a Simple Transient Gene Expression System in Tomato Fruit Protoplasts
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使用简单的瞬时基因表达系统分析番茄果实原生质体中的抗坏血酸生物合成

DOI:
10.1271/bbb.120844
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发表时间:
2013
期刊:
Bioscience, Biotechnology, and Biochemistry
影响因子:
--
通讯作者:
M. Esaka
M. Esaka
中科院分区:
--
文献类型:
--
作者:
S. Sakamoto;Y. Fujikawa;M. Esaka

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我们建立了一种利用番茄果实原生质体瞬时表达的新方法。该方法通过瞬时表达L-1-磷酸半乳糖磷酸酶基因,提高了番茄原生质体中L-抗坏血酸(AsA)含量。该系统为阐明番茄果实中AsA生物合成酶的功能和AsA的作用提供了快速分析手段。
We established a new method of transient expression using tomato fruit protoplasts. The method showed that L-ascorbic acid (AsA) content in tomato protoplasts was increased by transient expression of the L-galactose-1-phosphate phosphatase gene. This system provides a means of rapid analysis to clarify the function of AsA biosynthetic enzymes and AsA roles in tomato fruit.
DOI: 10.1093/pcp/pcm164
发表时间: 2008-01-01
影响因子: 4.9
作者:
Badejo, Adebanjo A.;Tanaka, Nobukazu;Esaka, Muneharu
通讯作者: Esaka, Muneharu