Multichannel resonant acoustic rheometry system for quantification of coagulation of multiple human plasma samples.
Multichannel resonant acoustic rheometry system for quantification of coagulation of multiple human plasma samples.
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DOI：
10.1038/s41598-023-46518-w
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发表时间：
2023-11-07
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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共振声流变仪（RAR）是一种基于超声的非接触式软粘弹性材料表征技术，可用于真实的实时定量评价软生物材料的粘弹性变化，并可用于监测血液凝固过程。在这里，我们报告了一种新的，多通道RAR（mRAR）系统的开发，用于同时测量多个时间演变的样本和演示其用于监测多个小体积血浆样本的凝血。mRAR系统使用4个定制设计的5.0 MHz超声换能器阵列和一个新型电子驱动系统构建，该系统控制同步超声脉冲的生成，用于同时对多个样本进行真实的时间评估。作为mRAR系统操作的概念验证，我们使用合并的正常人血浆样本和来自接受华法林治疗的患者的抗凝血浆样本（具有一系列国际标准化比值（INR）值，作为具有不同凝血动力学的良好表征样本）进行了检测。我们的研究结果表明，同时跟踪的动态变化，在4个血浆样本触发的高岭土或组织因子实现了整个凝血过程。mRAR系统捕获了样本中的明显变化，并确定了与INR水平一致的参数，包括凝血开始时间和与最终凝块硬度相关的参数。本研究的数据证明了mRAR系统用于有效表征多个血浆样本的动力学凝血过程的可行性。
Resonant Acoustic Rheometry (RAR), a newly developed ultrasound-based technique for non-contact characterization of soft viscoelastic materials, has shown promise for quantitative viscoelastic assessment of temporally changing soft biomaterials in real time, and may be used to monitor blood coagulation process. Here, we report the development of a novel, multichannel RAR (mRAR) system for simultaneous measurements of multiple temporally evolving samples and demonstration of its use for monitoring the coagulation of multiple small-volume plasma samples. The mRAR system was constructed using an array of 4 custom-designed ultrasound transducers at 5.0 MHz and a novel electronic driving system that controlled the generation of synchronized ultrasound pulses for real time assessment of multiple samples simultaneously. As a proof-of-concept of the operation of the mRAR system, we performed tests using pooled normal human plasma samples and anti-coagulated plasma samples from patients treated with warfarin with a range of International Normalized Ratio (INR) values as well-characterized samples with different coagulation kinetics. Our results show that simultaneous tracking of dynamic changes in 4 plasma samples triggered by either kaolin or tissue factor was achieved for the entire duration of coagulation. The mRAR system captured distinct changes in the samples and identified parameters including the clotting start time and parameters associated with the stiffness of the final clots that were consistent with INR levels. Data from this study demonstrate the feasibility of the mRAR system for efficient characterization of the kinetic coagulation processes of multiple plasma samples.
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影响因子：
4
作者：
Franchi, Francesco;Hammad, Jafri Syed;Angiolillo, Dominick J.
通讯作者：
Angiolillo, Dominick J.
影响因子：
3.1
作者：
Dunham, C. Michael;Rabel, Charlene;Hoffman, David A.
通讯作者：
Hoffman, David A.
影响因子：
5.7
作者：
Li, Weiping;Hobson, Eric C.;Deng, Cheri X.
通讯作者：
Deng, Cheri X.
DOI：
10.1109/tuffc.2017.2739649
发表时间：
2017-10
期刊：
IEEE transactions on ultrasonics, ferroelectrics, and frequency control
影响因子：
--
作者：
Maxwell AD;Yuldashev PV;Kreider W;Khokhlova TD;Schade GR;Hall TL;Sapozhnikov OA;Bailey MR;Khokhlova VA
通讯作者：
Khokhlova VA
影响因子：
3.9
作者：
通讯作者：
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