Requirement of the juxtamembrane domain of the cadherin cytoplasmic tail for morphogenetic cell rearrangement during myotome development.
Requirement of the juxtamembrane domain of the cadherin cytoplasmic tail for morphogenetic cell rearrangement during myotome development.
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DOI：
10.1083/jcb.200108156
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发表时间：
2001-12-24
期刊：
影响因子：
--
通讯作者：
Takeichi M
中科院分区：
文献类型：
--
作者：
Horikawa K;Takeichi M
在发育过程中，钙粘蛋白细胞粘附分子的活性被认为是受调节的，以允许细胞重排或易位。以往的研究表明，钙粘蛋白胞质尾区的跨膜（JM）结构域，其中包含的网站结合p120 ctn，在这个粘附系统中具有调节功能。为了研究JM结构域依赖的钙粘蛋白调控在胚胎细胞重排中的可能作用，我们在鸡胚体节发育过程中异位表达了一系列N-钙粘蛋白突变体。当JM结构域缺陷的N-钙粘蛋白表达时，肌节的形态发生扩张受到强烈抑制。然而，JM结构域中的三重丙氨酸取代，特异性抑制p120 ctn结合，对肌节发育没有影响。此外，显性负N-钙粘蛋白，在细胞外结构域的缺失，但保持正常的胞质尾，不影响肌节扩张，虽然它破坏了intersomite边界。p120 ctn的过表达也没有影响肌节扩张，但它扰乱肌纤维的方向。这些和其他的观察结果表明，JM结构域的N-钙粘蛋白有一个调节作用，在肌节细胞重排中的分子以外的p120 ctn参与。p120 ctn分子本身似乎在肌纤维的排列中起着关键作用。
During development, the activity of cadherin cell adhesion molecules is assumed to be regulated to allow for cell rearrangement or translocation. Previous studies suggest that the juxtamembrane (JM) domain of the cadherin cytoplasmic tail, which contains the site for binding to p120ctn, has a regulatory function in this adhesion system. To study the possible role of JM domain–dependent cadherin regulation in embryonic cell rearrangement, we ectopically expressed a series of N-cadherin mutants in developing somites of chicken embryos. When a JM domain–deficient N-cadherin was expressed, the morphogenetic expansion of the myotome was strongly suppressed. However, a triple alanine substitution in the JM domain, which specifically inhibited the p120ctn binding, had no effect on myotome development. Furthermore, a dominant negative N-cadherin, which had a deletion at the extracellular domain but maintained the normal cytoplasmic tail, did not affect myotome expansion; although it disrupted intersomite boundaries. Overexpression of p120ctn also did not affect myotome expansion, but it did perturb myofiber orientation. These and other observations suggest that the JM domain of N-cadherin has a regulatory role in myotome cell rearrangement in which molecules other than p120ctn are involved. The p120ctn molecule itself seems to play a critical role in the arrangement of myofibers.
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影响因子：
3.6
作者：
Kalcheim, C;Cinnamon, Y;Kahane, N
通讯作者：
Kahane, N
影响因子：
4.8
作者：
Ohkubo, T;Ozawa, M
通讯作者：
Ozawa, M
DOI：
10.1083/jcb.126.2.519
发表时间：
1994-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brieher WM;Gumbiner BM
通讯作者：
Gumbiner BM
影响因子：
3.3
作者：
FUJIMORI, T;TAKEICHI, M
通讯作者：
TAKEICHI, M
影响因子：
2.7
作者：
LEE, CH;GUMBINER, BM
通讯作者：
GUMBINER, BM