Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.)
Analysis of gene expression profiles of two near-isogenic lines differing at a QTL region affecting oil content at high temperatures during seed maturation in oilseed rape (Brassica napus L.)
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油菜种子成熟过程中高温下影响油含量的 QTL 区域不同的两个近等基因系的基因表达谱分析（甘蓝型油菜）
DOI：
10.1007/s00122-011-1725-2
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发表时间：
2012-02
影响因子：
5.4
通讯作者：
Jiang LX
中科院分区：
文献类型：
--
作者：
Zhu J;Meng JL;Zou JT;Jiang LX
油菜籽油产量受种子成熟过程中温度的影响很大。然而，种子成熟过程中基因型与温度之间相互作用的分子机制仍然很大程度上未知。我们开发了两个近等基因系（NIL-9和NIL-1），其差异主要在于在种子成熟过程的高温下影响甘蓝型油菜染色体C2（qOC.C2.2）含油量的QTL区域。开花后，NIL 在生长室中于不同温度下进行处理。开花后第 25 天 (DAF) 从发育中的种子中提取 RNA，并通过微阵列分析测定转录组。统计分析表明，基因型、温度以及基因型与温度之间的相互作用（G×T）均显着影响25个DAF种子中基因的表达，分别产生4,982、19,111和839个差异表达单序列。在实验的所有温度下，尤其是在高温下，NIL-9的种子油含量均高于NIL-1。共有39个基因，其中6个位于qOC.C2.2，无论温度如何，NIL之间都有差异表达，表明核心遗传分歧不受温度影响。温度升高导致种子油含量降低，同时伴随着许多与红光反应、光合作用、赤霉酸刺激反应和翻译伸长相关的基因以及脂质代谢途径中重要的几个基因的下调。这些结果有助于我们了解 QTL 的分子性质以及基因型与温度之间的相互作用。
Seed oil production in oilseed rape is greatly affected by the temperature during seed maturation. However, the molecular mechanism of the interaction between genotype and temperature in seed maturation remains largely unknown. We developed two near-isogenic lines (NIL-9 and NIL-1), differing mainly at a QTL region influencing oil content onBrassica napuschromosome C2 (qOC.C2.2) under high temperature during seed maturation. The NILs were treated under different temperatures in a growth chamber after flowering. RNA from developing seeds was extracted on the 25th day after flowering (DAF), and transcriptomes were determined by microarray analysis. Statistical analysis indicated that genotype, temperature, and the interaction between genotype and temperature (G × T) all significantly affected the expression of the genes in the 25 DAF seeds, resulting in 4,982, 19,111, and 839 differentially expressed unisequences, respectively. NIL-9 had higher seed oil content than NIL-1 under all of the temperatures in the experiments, especially at high temperatures. A total of 39 genes, among which six are located atqOC.C2.2, were differentially expressed among the NILs regardless of temperature, indicating the core genetic divergence that was unaffected by temperature. Increasing the temperature caused a reduction in seed oil content that was accompanied by the downregulation of a number of genes associated with red light response, photosynthesis, response to gibberellic acid stimulus, and translational elongation, as well as several genes of importance in the lipid metabolism pathway. These results contribute to our knowledge of the molecular nature of QTLs and the interaction between genotype and temperature.
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F-box蛋白家族。
影响因子：
12.3
作者：
Kipreos, E T;Pagano, M
通讯作者：
Pagano, M
DOI：
10.1139/b65-008
发表时间：
1965-01-01
期刊：
CANADIAN JOURNAL OF BOTANY
影响因子：
--
作者：
CANVIN, DT
通讯作者：
CANVIN, DT
影响因子：
3
作者：
Tchagang AB;Bui KV;McGinnis T;Benos PV
通讯作者：
Benos PV
影响因子：
5.1
作者：
Kachroo, Aardra;Shanklin, John;Kachroo, Pradeep
通讯作者：
Kachroo, Pradeep
影响因子：
5
作者：
Cao, Yujin;Yang, Jianming;Liu, Wei
通讯作者：
Liu, Wei