Oligosaccharide binding proteins from Bifidobacterium longum subsp. infantis reveal a preference for host glycans.
Oligosaccharide binding proteins from Bifidobacterium longum subsp. infantis reveal a preference for host glycans.
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DOI：
10.1371/journal.pone.0017315
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发表时间：
2011-03-15
期刊：
影响因子：
3.7
通讯作者：
Mills DA
中科院分区：
文献类型：
--
作者：
Garrido D;Kim JH;German JB;Raybould HE;Mills DA
长双歧杆菌（B.母乳低聚糖（HMO）是婴儿肠道微生物群的常见成员，其特征在于其对母乳低聚糖（HMO）的觅食能力。其基因组序列显示了过量的溶质结合蛋白（F1 SBPs）家族1，ABC转运蛋白的一部分，并与寡糖的进口。在这项研究中，我们使用哺乳动物聚糖阵列来确定这些蛋白质的特异性亲和力。这与包括HMO在内的不同益生元诱导的结合蛋白表达相关。B中的一半F1 SBPs。确定了结合哺乳动物寡糖的酶。它们的亲和力包括不同的血型结构和粘蛋白寡糖。与HMO相关，其他蛋白质对具有不同岩藻糖基化程度的乳糖-N-二糖（LNB）和聚乳糖胺的低聚物具有特异性。HMO上的生长诱导特异性结合蛋白的表达，这些蛋白进口HMO异构体，但也结合血型和粘蛋白寡糖，这表明共调节的运输机制。益生元菊粉诱导其他家族1结合蛋白与肠道聚糖的亲和力。大多数宿主聚糖F1 SBP在B中。在其他细菌中没有同源物。最后，这些蛋白中的一些被发现是粘附肠上皮细胞在体外。总之，这项研究为B的特殊适应提供了进一步的证据。这是对婴儿肠道环境的一种了解，有助于了解这一过程中所涉及的分子机制。
Bifidobacterium longum subsp. infantis (B. infantis) is a common member of the infant intestinal microbiota, and it has been characterized by its foraging capacity for human milk oligosaccharides (HMO). Its genome sequence revealed an overabundance of the Family 1 of solute binding proteins (F1SBPs), part of ABC transporters and associated with the import of oligosaccharides. In this study we have used the Mammalian Glycan Array to determine the specific affinities of these proteins. This was correlated with binding protein expression induced by different prebiotics including HMO. Half of the F1SBPs in B. infantis were determined to bind mammalian oligosaccharides. Their affinities included different blood group structures and mucin oligosaccharides. Related to HMO, other proteins were specific for oligomers of lacto-N-biose (LNB) and polylactosamines with different degrees of fucosylation. Growth on HMO induced the expression of specific binding proteins that import HMO isomers, but also bind blood group and mucin oligosaccharides, suggesting coregulated transport mechanisms. The prebiotic inulin induced other family 1 binding proteins with affinity for intestinal glycans. Most of the host glycan F1SBPs in B. infantis do not have homologs in other bifidobacteria. Finally, some of these proteins were found to be adherent to intestinal epithelial cells in vitro. In conclusion, this study represents further evidence for the particular adaptations of B. infantis to the infant gut environment, and helps to understand the molecular mechanisms involved in this process.
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影响因子：
5.6
作者：
Higgins, Melanie A.;Abbott, D. Wade;Boulanger, Martin J.;Boraston, Alisdair B.
通讯作者：
Boraston, Alisdair B.
影响因子：
4.4
作者：
Kitaoka, M;Tian, JS;Nishimoto, M
通讯作者：
Nishimoto, M
影响因子：
5.4
作者：
CROCIANI, F;ALLESANDRINI, A;BIAVATI, B
通讯作者：
BIAVATI, B
影响因子：
4.4
作者：
Barboza, Mariana;Sela, David A.;Lebrilla, Carlito B.
通讯作者：
Lebrilla, Carlito B.
DOI：
10.1016/0005-2760(75)90028-4
发表时间：
1975-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
KERANEN, A
通讯作者：
KERANEN, A