Diffusion Tensor Cardiac Magnetic Resonance Reveals Exosomes From Cardiosphere-Derived Cells Preserve Myocardial Fiber Architecture After Myocardial Infarction.
Diffusion Tensor Cardiac Magnetic Resonance Reveals Exosomes From Cardiosphere-Derived Cells Preserve Myocardial Fiber Architecture After Myocardial Infarction.
复制标题
弥散张量心脏磁共振揭示了心肌球衍生细胞的外泌体在心肌梗塞后保留心肌纤维结构。
DOI：
10.1016/j.jacbts.2017.09.005
复制
发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Nguyen CT;Dawkins J;Bi X;Marbán E;Li D
CDCEXO是RNA负载的纳米颗粒，可减少瘢痕形成，阻止不良重塑，并保护啮齿动物和猪MI后的心脏功能。CDCEXO对心肌纤维结构的治疗作用及其与保存心脏功能和减少瘢痕形成的关系尚不清楚。心肌梗死猪心肌内CDCEXO治疗后，DT-CMR阐明的心肌纤维结构得到保留，这表明螺旋角透壁性未发生变化。常规CMR测量的瘢痕大小与DT-CMR测量的螺旋角透壁性相结合，显示出对心功能预测的显著改善。DT-CMR是一种强大的心肌再生治疗评估技术，揭示了对心肌微观结构的独特见解。本研究的目的是通过弥散张量心脏磁共振成像揭示慢性猪心肌梗死中心肌内由心球衍生细胞分泌的外泌体（CDCEXO）后的纤维微结构反应。在一项随机安慰剂对照研究中，心肌梗死猪接受了人CDCEXO和安慰剂的心肌内给药。注射4周后，CDCEXO组的存活率得到改善，而心肌纤维结构和心脏功能得到保留。在安慰剂组，纤维结构和心脏功能下降。通过CDCEXO的心肌再生不是肿瘤样的;相反，组织结构的细节被忠实地保留，这可能促进生理兴奋和收缩。
CDCEXO are RNA-laden nanoparticles that reduce scarring, halt adverse remodeling, and preserve cardiac function in rodents and pigs after MI. The therapeutic effects of CDCEXO on myocardial fiber architecture and how it relates to preserved cardiac function and reduced scarring remain unclear. After intramyocardial CDCEXO treatment in MI pigs, DT-CMR elucidated myocardial fiber architecture was preserved indicated by the unchanged helix angle transmurality. Scar size measured by conventional CMR combined with helix angle transmurality measured by DT-CMR demonstrated significant improvement in the prediction of cardiac function. DT-CMR is a powerful technology for myocardial regenerative therapy evaluation revealing unique insight into the myocardium’s microstructure. The object of the study was to reveal the fiber microstructural response with diffusion tensor cardiac magnetic resonance after intramyocardial exosomes secreted by cardiosphere-derived cells (CDCEXO) in chronic porcine myocardial infarction. Porcine with myocardial infarction underwent intramyocardial delivery of human CDCEXO and placebo in a randomized placebo-controlled study. Four weeks after injection, viability improved in the CDCEXO group, whereas myocardial fiber architecture and cardiac function were preserved. In the placebo group, fiber architecture and cardiac function declined. Myocardial regeneration by CDCEXO is not tumor-like; instead, details of tissue architecture are faithfully preserved, which may foster physiological excitation and contraction.
登录
查看更多内容
DOI：
10.1186/1532-429x-14-70
发表时间：
2012-10-12
期刊：
Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance
影响因子：
--
作者：
Mekkaoui C;Huang S;Chen HH;Dai G;Reese TG;Kostis WJ;Thiagalingam A;Maurovich-Horvat P;Ruskin JN;Hoffmann U;Jackowski MP;Sosnovik DE
通讯作者：
Sosnovik DE
影响因子：
168.9
作者：
Makkar, Raj R.;Smith, Rachel R.;Cheng, Ke;Malliaras, Konstantinos;Thomson, Louise E. J.;Berman, Daniel;Czer, Lawrence S. C.;Marban, Linda;Mendizabal, Adam;Johnston, Peter V.;Russell, Stuart D.;Schuleri, Karl H.;Lardo, Albert C.;Gerstenblith, Gary;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
5.7
作者：
Khan AR;Cornea A;Leigland LA;Kohama SG;Jespersen SN;Kroenke CD
通讯作者：
Kroenke CD
影响因子：
3.5
作者：
Budde MD;Annese J
通讯作者：
Annese J
影响因子：
5
作者：
Fomovsky GM;Rouillard AD;Holmes JW
通讯作者：
Holmes JW