Differential gene expression associated with dietary methylmercury (MeHg) exposure in rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio).
Differential gene expression associated with dietary methylmercury (MeHg) exposure in rainbow trout (Oncorhynchus mykiss) and zebrafish (Danio rerio).
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DOI：
10.1007/s10646-013-1066-9
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发表时间：
2013-05
期刊：
影响因子：
2.7
通讯作者：
Carvan, Michael J., III
中科院分区：
文献类型：
--
作者：
Liu, Qing;Basu, Niladri;Goetz, Giles;Jiang, Nan;Hutz, Reinhold J.;Tonellato, Peter J.;Carvan, Michael J., III
本研究的目的是确定和评估保守的生物标志物，可以用于大多数种类的硬骨鱼在大多数生命阶段。我们研究了亚致死甲基汞（甲基汞）暴露对发育中的虹鳟鱼和斑马鱼的影响。幼虹鳟鱼和年轻的成年斑马鱼饲料中添加0，0.5，5和50 ppm的甲基汞。采用原子吸收光谱法测定全身总汞含量，并进行病理学分析，以确定甲基汞引起的毒性。从每组中取样六周的鱼，使用来自整条鱼的RNA进行微阵列分析。甲基汞暴露的鳟鱼和斑马鱼没有表现出明显的毒性或病理学迹象，也没有显着差异，死亡率，长度，质量，或条件因素。在六周内，鳟鱼和斑马鱼体内甲基汞的积累呈现出剂量和时间依赖性模式，斑马鱼对总汞的同化作用大于虹鳟鱼。在甲基汞处理的鱼的失调基因有多个功能注释，如铁离子稳态，谷胱甘肽转移酶活性，肌肉收缩的调节，肌钙蛋白I结合和钙依赖性蛋白结合。基于其微阵列数据选择基因作为生物标志物候选物，并通过QPCR评估其表达。不幸的是，这些基因不是虹鳟鱼和斑马鱼的良好一致的生物标志物，来自使用个体鱼的QPCR评估。我们的结论是，生物标志物分析水生毒物评估使用鱼类需要基于组织，性别和物种的具体考虑。
The objective of this study was to identify and evaluate conserved biomarkers that could be used in most species of teleost fish at most life-stages. We investigated the effects of sublethal methylmercury (MeHg) exposure on developing rainbow trout and zebrafish. Juvenile rainbow trout and young adult zebrafish were fed food with MeHg added at 0, 0.5, 5 and 50 ppm. Atomic absorption spectrometry was applied to measure whole body total Hg levels, and pathologic analysis was performed to identify MeHg-induced toxicity. Fish at six weeks were sampled from each group for microarray analysis using RNA from whole fish. MeHg-exposed trout and zebrafish did not show overt signs of toxicity or pathology, nor were significant differences seen in mortality, length, mass, or condition factor. The accumulation of MeHg in trout and zebrafish exhibited dose- and time-dependent patterns during six weeks, and zebrafish exhibited greater assimilation of total Hg than rainbow trout. The dysregulated genes in MeHg-treated fish have multiple functional annotations, such as iron ion homeostasis, glutathione transferase activity, regulation of muscle contraction, troponin I binding and calcium-dependent protein binding. Genes were selected as biomarker candidates based on their microarray data and their expression was evaluated by QPCR. Unfortunately, these genes are not good consistent biomarkers for both rainbow trout and zebrafish from QPCR evaluation using individual fish. Our conclusion is that biomarker analysis for aquatic toxicant assessment using fish needs to be based on tissue-, sex- and species-specific consideration.
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影响因子：
4.4
作者：
Gunnarsson L;Kristiansson E;Förlin L;Nerman O;Larsson DG
通讯作者：
Larsson DG
影响因子：
4.5
作者：
GIBLIN, FJ;MASSARO, EJ
通讯作者：
MASSARO, EJ
影响因子：
4.6
作者：
Lewis, Johanne M.;Hori, Tiago S.;Currie, Suzanne
通讯作者：
Currie, Suzanne
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
4
作者：
Benoit, JM;Gilmour, CC;Riedel, GF
通讯作者：
Riedel, GF