Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides

Intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides
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人副流感3型病毒特异多肽的细胞内合成

DOI:
10.1128/jvi.54.3.661-664.1985
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发表时间:
1985
影响因子:
5.4
通讯作者:
M. Pons
M. Pons
中科院分区:
医学2区
文献类型:
--
作者:
S. Wechsler;D. Lambert;M. Galinski;M. Pons

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在还原条件下,用[35S]蛋氨酸标记的细胞提取物的聚丙烯酰胺凝胶电泳法检测了人副流感病毒3型特异性多肽的细胞内合成。在细胞提取液中均检测到病毒粒子结构蛋白,包括:L,180K;P,83K;HN,69K;NP,66K;F0,60K;F1,51K;M,38K。P和NP被磷酸化。HN和F是糖基化的。细胞内病毒蛋白合成动力学未检测到任何早期或晚期蛋白。脉冲追逐实验未能检测到任何前体与产物之间的关系。未检测到非结构蛋白。
The intracellular synthesis of human parainfluenza type 3 virus-specified polypeptides was examined by polyacrylamide gel electrophoresis of [35S]methionine-labeled cell extracts under reducing conditions. All of the virion structural proteins were detected in cell extracts, including: L, 180,000 molecular weight (180K); P, 83K; HN, 69K; NP, 66K; F0, 60K; F1, 51K; and M, 38K. P and NP were phosphorylated. HN and F were glycosylated. The kinetics of intracellular viral protein synthesis did not detect any early or late proteins. Pulse-chase experiments failed to detect any precursor-product relationships. No nonstructural proteins were detected.
儿童时期副流感病毒感染的自然史。
DOI: 10.1016/s0022-3476(82)80113-3
发表时间: 1982
期刊: The Journal of pediatrics
影响因子: --
作者:
Welliver,R;Wong,DT;Choi,TS;Ogra,PL
通讯作者: Ogra,PL