Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus Detected in African Blue Ticks Feeding on Cattle in a Ugandan Abattoir.
Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus Detected in African Blue Ticks Feeding on Cattle in a Ugandan Abattoir.
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DOI：
10.3390/microorganisms9020438
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发表时间：
2021-02-20
期刊：
影响因子：
4.5
通讯作者：
Stubbs SCB
中科院分区：
文献类型：
--
作者：
Wampande EM;Waiswa P;Allen DJ;Hewson R;Frost SDW;Stubbs SCB
克里米亚-刚果出血热病毒（CCHFV）是地理分布最广的蜱传病毒。然而，非洲株CCHFV在序列数据库中的代表性很差。此外，迄今为止收集的几乎所有序列数据都是从人类疾病病例中获得的，而有关病毒在蜱虫和动物宿主中传播的信息严重缺乏。在这里，我们描述了一种新的CCHFV株的完整编码区，检测到非洲蓝蜱（Rhipicephalus（Boophilus）decoloratus）饲养牛在坎帕拉，乌干达的屠宰场。这些牛来自姆巴拉拉的一个农场，姆巴拉拉是乌干达大部分地区的主要牲畜贸易中心。系统发育分析表明，新测序的菌株属于非洲基因型II分支，其中主要包含20世纪50年代从西非分离的菌株序列，以及20世纪80年代从南非分离的菌株序列。虽然病毒S（核蛋白）和L（RNA聚合酶）基因组片段与先前报道的基因型II毒株具有>90%的核苷酸相似性，但糖蛋白编码M片段与下一个最密切相关的毒株仅具有80%的核苷酸相似性，这些毒株来自印度西部和北方中国的蜱。该基因组片段还显示了大量的非同义突变，以前未报告的基因型II菌株。这种新菌株的特性增加了我们对蜱和非洲CCHFV自然多样性的有限理解。这些数据可用于为疫苗和诊断的设计提供信息，以及探索该病毒的流行病学和演变的研究，以建立未来的CCHFV控制策略。
Crimean-Congo hemorrhagic fever virus (CCHFV) is the most geographically widespread of the tick-borne viruses. However, African strains of CCHFV are poorly represented in sequence databases. In addition, almost all sequence data collected to date have been obtained from cases of human disease, while information regarding the circulation of the virus in tick and animal reservoirs is severely lacking. Here, we characterize the complete coding region of a novel CCHFV strain, detected in African blue ticks (Rhipicephalus (Boophilus) decoloratus) feeding on cattle in an abattoir in Kampala, Uganda. These cattle originated from a farm in Mbarara, a major cattle-trading hub for much of Uganda. Phylogenetic analysis indicates that the newly sequenced strain belongs to the African genotype II clade, which predominantly contains the sequences of strains isolated from West Africa in the 1950s, and South Africa in the 1980s. Whilst the viral S (nucleoprotein) and L (RNA polymerase) genome segments shared >90% nucleotide similarity with previously reported genotype II strains, the glycoprotein-coding M segment shared only 80% nucleotide similarity with the next most closely related strains, which were derived from ticks in Western India and Northern China. This genome segment also displayed a large number of non-synonymous mutations previously unreported in the genotype II strains. Characterization of this novel strain adds to our limited understanding of the natural diversity of CCHFV circulating in both ticks and in Africa. Such data can be used to inform the design of vaccines and diagnostics, as well as studies exploring the epidemiology and evolution of the virus for the establishment of future CCHFV control strategies.
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影响因子：
6.7
作者：
Fusco ML;Hashiguchi T;Cassan R;Biggins JE;Murin CD;Warfield KL;Li S;Holtsberg FW;Shulenin S;Vu H;Olinger GG;Kim DH;Whaley KJ;Zeitlin L;Ward AB;Nykiforuk C;Aman MJ;Berry JD;Saphire EO
通讯作者：
Saphire EO
影响因子：
10.7
作者：
Minh BQ;Nguyen MA;von Haeseler A
通讯作者：
von Haeseler A
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ
影响因子：
9.4
作者：
Lambert, Amy J.;Lanciotti, Robert S.
通讯作者：
Lanciotti, Robert S.
影响因子：
5
作者：
Sahay, Rima R.;Dhandore, Suhas;Gopalakrishnan, Maya
通讯作者：
Gopalakrishnan, Maya