(1)H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth.
(1)H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth.
复制标题
（1）1H-NMR代谢组学研究揭示了海葫芦乙酸乙酯提取物抑制肝癌细胞生长的多靶点作用。
DOI：
10.1038/s41598-020-78867-1
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发表时间：
2021-01-13
影响因子：
4.6
通讯作者：
Mazzocca A
中科院分区：
文献类型：
--
作者：
Gnocchi D;Del Coco L;Girelli CR;Castellaneta F;Cesari G;Sabbà C;Fanizzi FP;Mazzocca A
肝细胞癌（HCC）目前是全球肿瘤相关疾病的第六大原因，估计到2030年将成为西方国家的第三大原因。治疗肝癌的新药仍有许多不良反应。一些证据表明，植物代谢物为开发包括癌症在内的许多疾病的新治疗策略提供了具体的机会。我们以前报道过，乙酸乙酯提取物的自发食用植物收获的普利亚，海滨，显着抑制肝癌细胞的细胞生长。通过1H-NMR光谱，在这里，我们表明，Crithmum maritimum乙酸乙酯提取物抵消的瓦尔堡效应，通过减少细胞内乳酸，抑制蛋白质的合成，通过降低氨基酸水平，并影响膜生物合成，通过降低胆碱和磷酸胆碱。此外，我们观察到对脂质稳态的影响，甘油三酯、胆固醇、单不饱和脂肪酸（MUFA）和双不饱和脂肪酸（DUFA）减少，多不饱和脂肪酸（PUFA）增加。综上所述，这些数据表明，Crithmum maritimum诱导的细胞抑制作用是通过多效应作用发挥的，靶向HCC细胞中的关键代谢过程。总的来说，我们的研究结果强调了Crithmum maritimum作为预防和改善HCC和其他类型肿瘤治疗选择的有前途的工具的作用。
Hepatocellular carcinoma (HCC) is nowadays the sixth cause of tumour-related deceases worldwide, estimated to become the third in Western countries by 2030. New drugs for HCC treatment still have many adverse effects. Several lines of evidence indicate that plant metabolites offer concrete opportunities for developing new therapeutic strategies for many diseases, including cancer. We previously reported that ethyl acetate extract of a spontaneous edible plant harvested in Apulia, Crithmum maritimum, significantly inhibited cell growth in HCC cells. By 1H-NMR spectroscopy, here we show that Crithmum maritimum ethyl acetate extract counteracts the Warburg effect, by reducing intracellular lactate, inhibits protein anabolism, by decreasing amino acid level, and affects membrane biosynthesis by lowering choline and phosphocholine. Also, we observed an effect on lipid homeostasis, with a reduction in triglycerides, cholesterol, monounsaturated fatty acids (MUFA), and diunsaturated fatty acids (DUFA), and an increase in polyunsaturated fatty acids (PUFA). Taken together, these data demonstrate that Crithmum maritimum-induced cytostasis is exerted through a multi-effect action, targeting key metabolic processes in HCC cells. Overall, our findings highlight the role of Crithmum maritimum as a promising tool for the prevention and the improvement of the therapeutic options for HCC and other types of tumours.
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将植物化学物质和植物疗法整合到癌症精确医学中。
影响因子：
--
作者：
Efferth T;Saeed MEM;Mirghani E;Alim A;Yassin Z;Saeed E;Khalid HE;Daak S
通讯作者：
Daak S
影响因子：
6
作者：
Dona AC;Kyriakides M;Scott F;Shephard EA;Varshavi D;Veselkov K;Everett JR
通讯作者：
Everett JR
影响因子：
5.4
作者：
Gu, Liang;Saha, Sourav Taru;Kaur, Mandeep
通讯作者：
Kaur, Mandeep
影响因子：
5.2
作者：
Casadei-Gardini, Andrea;Del Coco, Laura;Giudetti, Anna Maria
通讯作者：
Giudetti, Anna Maria
影响因子：
4.2
作者：
Chen, Xu-Zheng;Cao, Zhi-Yun;Du, Jian
通讯作者：
Du, Jian