The Drug H(+) Antiporter FgQdr2 Is Essential for Multiple Drug Resistance, Ion Homeostasis, and Pathogenicity in Fusarium graminearum.
The Drug H(+) Antiporter FgQdr2 Is Essential for Multiple Drug Resistance, Ion Homeostasis, and Pathogenicity in Fusarium graminearum.
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药物 H 反向转运蛋白 FgQdr2 对于禾谷镰刀菌的多重耐药性、离子稳态和致病性至关重要
DOI：
10.3390/jof8101009
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发表时间：
2022-09-26
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影响因子：
--
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中科院分区：
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--
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越来越多的抗药性和DON污染给镰刀菌赤霉病的防治带来了严重的问题。虽然H+逆向转运蛋白(DHA)家族在耐药中起着关键作用，但在病原性真菌中DHA转运蛋白的特性还没有得到系统的研究。本研究对禾谷镰刀菌中所有可能的DHA转运蛋白基因进行了系统的缺失分析，发现其中一个DHA1转运蛋白FgQdr2与多重耐药、离子动态平衡和毒力有关。进一步的研究表明，FgQdr2主要定位于细胞膜，在正常生长条件下其表达水平较低，但足以调节药物外排。此外，对其生理底物的研究表明，FgQdr2对于K+、Na+、Cu2+的运输和膜质子梯度的调节是必不可少的。FgQdr2在赤霉病病程中的作用是通过调节毒力因子脱氧雪腐镰刀菌烯醇(DON)的生物合成、植物抗毒素的清除以及禾谷镰刀菌的无性繁殖和有性繁殖而与真菌感染有关。总之，本研究结果揭示了FgQdr2在多重耐药、离子稳态和致病性中的重要作用，从而加深了对致病真菌中DHA转运蛋白的理解。
Increased emergence of drug resistance and DON pollution pose a severe problem in Fusarium head blight (FHB) control. While the H+ antiporter (DHA) family plays crucial roles in drug resistance, the characterization of DHA transporters has not been systematically studied in pathogenetic fungi. In this study, a systematic gene deletion analysis of all putative DHA transporter genes was carried out in Fusarium graminearum, and one DHA1 transporter FgQdr2 was found to be involved in multiple drug resistance, ion homeostasis, and virulence. Further exploration showed that FgQdr2 is mainly localized in the cell membrane; its expression under normal growth conditions is comparatively low, but sufficient for the regulation of drug efflux. Additionally, investigation of its physiological substrates demonstrated that FgQdr2 is essential for the transport of K+, Na+, Cu2+, and the regulation of the membrane proton gradient. For its roles in the FHB disease cycle, FgQdr2 is associated with fungal infection via regulating the biosynthesis of virulence factor deoxynivalenol (DON), the scavenging of the phytoalexin, as well as both asexual and sexual reproduction in F. graminearum. Overall, the results of this study reveal the crucial roles of FgQdr2 in multiple drug resistance, ion homeostasis, and pathogenicity, which advance the understanding of the DHA transporters in pathogenetic fungi.
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影响因子：
4.9
作者：
Kettle, Andrew J.;Batley, Jacqueline;Gardiner, Donald M.
通讯作者：
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影响因子：
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DOI：
10.1007/978-3-211-49389-2_2
发表时间：
2007-01-01
期刊：
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影响因子：
--
作者：
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通讯作者：
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作者：
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