Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat.
Major Facilitator Superfamily Transporter Gene FgMFS1 Is Essential for Fusarium graminearum to Deal with Salicylic Acid Stress and for Its Pathogenicity towards Wheat.
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DOI：
10.3390/ijms22168497
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发表时间：
2021-08-07
影响因子：
5.6
通讯作者：
Qi P
中科院分区：
文献类型：
--
作者：
Chen Q;Lei L;Liu C;Zhang Y;Xu Q;Zhu J;Guo Z;Wang Y;Li Q;Li Y;Kong L;Jiang Y;Lan X;Wang J;Jiang Q;Chen G;Ma J;Wei Y;Zheng Y;Qi P
小麦是世界上主要的粮食作物，由于其总产量和独特的加工品质。小麦赤霉病主要由禾谷镰刀菌（Fusarium graminearum）引起，严重威胁小麦的产量和品质。水杨酸（SA）对F.禾谷镰刀菌是一种有希望的可持续控制对象。F.禾本科植物能够有效地应对SA胁迫。然而，其潜在机制仍不清楚。在此，我们对F.禾谷早熟禾FgMFS1在感染过程中高度表达，并被SA上调。预测的FgMFS1蛋白的三维结构与反向转运蛋白的示意图一致。亚细胞定位实验表明，FgMFS1通常在菌丝的液泡中表达，但在SA处理下，FgMFS1交替分布在细胞膜上，表明FgMFS1是F. graminearum对SA.ΔFgMFS1（FgMFS1的功能缺失突变体）对SA的敏感性增强，对小麦的致病性降低，并在SA胁迫下减少DON的产生。将功能性FgMFS1基因重新引入到FgMFS1中恢复了突变体表型。接种ΔFgMFS1的小麦穗比接种野生型菌株的穗积累更多的SA。FgMFS1在酵母中的异位表达如预期的那样增强了其对SA的耐受性，进一步证明FgMFS1作为SA输出者起作用。总之，FgMFS1在F.禾谷镰刀菌（graminearum）对小麦内源SA的反应和对小麦的致病性起着关键作用。
Wheat is a major staple food crop worldwide, due to its total yield and unique processing quality. Its grain yield and quality are threatened by Fusarium head blight (FHB), which is mainly caused by Fusarium graminearum. Salicylic acid (SA) has a strong and toxic effect on F. graminearum and is a hopeful target for sustainable control of FHB. F. graminearum is capable of efficientdealing with SA stress. However, the underlying mechanisms remain unclear. Here, we characterized FgMFS1 (FGSG_03725), a major facilitator superfamily (MFS) transporter gene in F. graminearum. FgMFS1 was highly expressed during infection and was upregulated by SA. The predicted three-dimensional structure of the FgMFS1 protein was consistent with the schematic for the antiporter. The subcellular localization experiment indicated that FgMFS1 was usually expressed in the vacuole of hyphae, but was alternatively distributed in the cell membrane under SA treatment, indicating an element of F. graminearum in response to SA. ΔFgMFS1 (loss of function mutant of FgMFS1) showed enhanced sensitivity to SA, less pathogenicity towards wheat, and reduced DON production under SA stress. Re-introduction of a functional FgMFS1 gene into ∆FgMFS1 recovered the mutant phenotypes. Wheat spikes inoculated with ΔFgMFS1 accumulated more SA when compared to those inoculated with the wild-type strain. Ecotopic expression of FgMFS1 in yeast enhanced its tolerance to SA as expected, further demonstrating that FgMFS1 functions as an SA exporter. In conclusion, FgMFS1 encodes an SA exporter in F. graminearum, which is critical for its response to wheat endogenous SA and pathogenicity towards wheat.
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影响因子：
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Ledbetter, Eric C.;Norman, Mary L.;Starr, Jennifer K.
通讯作者：
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