A human SARS-CoV neutralizing antibody against epitope on S2 protein.
A human SARS-CoV neutralizing antibody against epitope on S2 protein.
复制标题
针对 S2 蛋白表位的人类 SARS-CoV 中和抗体。
DOI：
10.1016/j.bbrc.2005.05.089
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发表时间：
2005-07-22
影响因子：
3.1
通讯作者：
Jin G
中科院分区：
文献类型：
--
作者：
Duan J;Yan X;Guo X;Cao W;Han W;Qi C;Feng J;Yang D;Gao G;Jin G
利用SARS恢复期患者B细胞构建免疫抗体噬菌体展示文库。以SARS-CoV全灭活病毒体为靶，从文库中筛选出80多个克隆。一种人类scFv B1被广泛表征。B1在体内识别SARS假病毒，并与SARS血清竞争，以高亲和力与SARS冠状病毒结合（平衡解离常数Kd = 105 nM）。B1在体外对表达SARS-CoV S蛋白的假病毒感染也具有有效的中和活性。最后，我们发现B1在S2蛋白上识别一个表位，特别是在S2蛋白的1023-1189氨基酸内。本研究不仅首次制备出与血清中天然抗体一样识别S2蛋白表位的人中和抗体，而且可能有助于我们更好地了解SARS蛋白的免疫学特性和SARS疫苗设计。
An immune antibody phage-display library was constructed from B cells of SARS convalescent patients. More than 80 clones were selected from the library by using the whole inactivated SARS-CoV virions as target. One human scFv, B1, was characterized extensively. The B1 recognized SARS pseudovirus in vivo and competed with SARS sera for binding to SARS-CoV with high affinity (equilibrium dissociation constant, Kd = 105 nM). The B1 also has potent neutralizing activities against infection by pseudovirus expressing SARS-CoV S protein in vitro. Finally, we found that the B1 recognized an epitope on S2 protein, especially within amino acids 1023–1189 of S2 protein. This study not only first made a human neutralizing antibody, which recognized an epitope on S2 protein like natural antibody in sera, but also may help us to better understand the immunological characteristics of SARS protein and SARS vaccine design.
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影响因子：
64.8
作者：
Li W;Moore MJ;Vasilieva N;Sui J;Wong SK;Berne MA;Somasundaran M;Sullivan JL;Luzuriaga K;Greenough TC;Choe H;Farzan M
通讯作者：
Farzan M
DOI：
10.1016/s0140-6736(03)13077-2
发表时间：
2003-04-19
期刊：
Lancet (London, England)
影响因子：
--
作者：
Peiris JS;Lai ST;Poon LL;Guan Y;Yam LY;Lim W;Nicholls J;Yee WK;Yan WW;Cheung MT;Cheng VC;Chan KH;Tsang DN;Yung RW;Ng TK;Yuen KY;SARS study group
通讯作者：
SARS study group
DOI：
10.1007/s10096-004-1271-9
发表时间：
2005-01
期刊：
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
影响因子：
--
作者：
Cheng Y;Wong R;Soo YO;Wong WS;Lee CK;Ng MH;Chan P;Wong KC;Leung CB;Cheng G
通讯作者：
Cheng G
影响因子：
9.3
作者：
Choy, WY;Lin, SG;Ngai, SM
通讯作者：
Ngai, SM
影响因子：
3.7
作者：
MALMBORG, AC;MICHAELSSON, A;BORREBAECK, CAK
通讯作者：
BORREBAECK, CAK