The host niches of soybean rather than genetic modification or glyphosate application drive the assembly of root-associated microbial communities.
The host niches of soybean rather than genetic modification or glyphosate application drive the assembly of root-associated microbial communities.
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大豆的宿主生态位而不是基因改造或草甘膦应用驱动了根部相关微生物群落的组装
DOI：
10.1111/1751-7915.14164
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发表时间：
2022-12
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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作者：
植物根系对土壤微生物多样性有重要影响，土壤微生物在自然生态系统和农业生态系统中发挥着重要作用。尽管具有增强的抗虫性和抗除草剂性的转基因作物被认为具有无与伦比的产量和抗逆性优势，但由于转基因植物对土壤微生物群落的潜在影响，仍需要在真实的世界环境中进行全面深入的案例研究。在这项研究中，使用了三种处理：受体大豆品种Jack，三重转基因大豆品系JD 321和草甘膦处理的JD 321（JD 321 G）。本研究建立了大豆根际3个取样期（开花期、灌浆期和成熟期）和3个寄主生态位（完整根（RT）、根际土壤（RS）和周围土壤（SS））。与Jack相比，JD 321 G根际土壤在开花期具有较高的脲酶活性和较低的亚硝酸还原酶活性。不同处理和不同采样阶段对不同分类层次的微生物群落组成无显著影响。在属水平上，从根内到根外，被孢霉属（Mortierella）、毛壳菌属（Chaetomium）和拟嗜热假单胞菌属（Pseudombrophila）的相对丰度逐渐增加，而内生细菌黄杆菌属（Chryseobacterium）和病原细菌链霉菌属（Streptomyces）的相对丰度逐渐减少（即RT → RS → SS）。此外，两个细菌属，慢生根瘤菌和Ensifer在RT中比RS和SS中更丰富，以及三个物种，辐射土壤杆菌，Ensifer fredii和Ensifer meliloti，它们与固氮密切相关。此外，与固氮基因相关的五个邻位组（COG）簇在RT中高于RS，而只有一个注释为二氮酶铁钼辅因子生物合成蛋白的COG较低。总的来说，这些结果表明，整个土壤-植物连续体的根际宿主生态位在很大程度上控制了三重转基因大豆根相关微生物组的组成和功能。
Plant roots significantly influence soil microbial diversity, and soil microorganisms play significant roles in both natural and agricultural ecosystems. Although the genetically modified (GM) crops with enhanced insect and herbicide resistance are thought to have unmatched yield and stress resistance advantages, thorough and in‐depth case studies still need to be carried out in a real‐world setting due to the potential effects of GM plants on soil microbial communities. In this study, three treatments were used: a recipient soybean variety Jack, a triple transgenic soybean line JD321, and the glyphosate‐treated JD321 (JD321G). Three sampling stages (flowering, seed filling and maturing), as well as three host niches of soybean rhizosphere [intact roots (RT), rhizospheric soil (RS) and surrounding soil (SS)] were established. In comparison to Jack, the rhizospheric soil of JD321G had higher urease activity and lower nitrite reductase at the flowering stage. Different treatments and different sampling stages existed no significant effects on the compositions of microbial communities at different taxonomic levels. However, at the genus level, the relative abundance of three plant growth‐promoting fungal genera (i.e. Mortierella, Chaetomium and Pseudombrophila) increased while endophytic bacteria Chryseobacterium and pathogenic bacteria Streptomyces decreased from the inside to the outside of the roots (i.e. RT → RS → SS). Moreover, two bacterial genera, Bradyrhizobium and Ensifer were more abundant in RT than in RS and SS, as well as three species, Agrobacterium radiobacter, Ensifer fredii and Ensifer meliloti, which are closely related to nitrogen‐fixation. Furthermore, five clusters of orthologous groups (COGs) associated to nitrogen‐fixation genes were higher in RT than in RS, whereas only one COG annotated as dinitrogenase iron‐molybdenum cofactor biosynthesis protein was lower. Overall, the results imply that the rhizosphere host niches throughout the soil–plant continuum largely control the composition and function of the root‐associated microbiome of triple transgenic soybean.
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影响因子：
--
作者：
Galardini M;Bazzicalupo M;Biondi E;Brambilla E;Brilli M;Bruce D;Chain P;Chen A;Daligault H;Davenport KW;Deshpande S;Detter JC;Goodwin LA;Han C;Han J;Huntemann M;Ivanova N;Klenk HP;Kyrpides NC;Markowitz V;Mavrommatis K;Mocali S;Nolan M;Pagani I;Pati A;Pini F;Pitluck S;Spini G;Szeto E;Teshima H;Woyke T;Mengoni A
通讯作者：
Mengoni A
影响因子：
4.1
作者：
Hansen, Karen;Perry, Brian A.;Pfister, Donald H.
通讯作者：
Pfister, Donald H.
影响因子：
2.4
作者：
Dunfield, KE;Germida, JJ
通讯作者：
Germida, JJ
影响因子：
2.1
作者：
Bellato, CM;Pueppke, SG;Krishnan, HB
通讯作者：
Krishnan, HB
影响因子：
5.1
作者：
Espinosa-Urgel, Manuel
通讯作者：
Espinosa-Urgel, Manuel