vanI: a novel D-Ala-D-Lac vancomycin resistance gene cluster found in Desulfitobacterium hafniense.
vanI: a novel D-Ala-D-Lac vancomycin resistance gene cluster found in Desulfitobacterium hafniense.
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DOI：
10.1111/1751-7915.12139
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发表时间：
2014-09
影响因子：
5.7
通讯作者：
Smidt H
中科院分区：
文献类型：
--
作者：
Kruse T;Levisson M;de Vos WM;Smidt H
直到最近，糖肽万古霉素还被认为是对抗革兰氏阳性细菌的最后手段。然而，越来越多的细菌被发现携带对这种抗生素产生耐药性的基因。到目前为止，已经描述了10种不同的万古霉素耐药簇。染色体万古霉素耐药基因簇先前被描述为厌氧Desulfitobacterium hafniense Y51。结果表明，该基因簇以编码vanI基因的d-Ala-d-Lac连接酶为特征，存在于所有hafniense D.菌株、chlororespirans D.菌株和Desulfosporosinus spp.菌株中，而在对万古霉素敏感的Desulfitobacterium或desulfosporosinspp .菌株中未发现该基因簇，该基因簇可作为hafniense D.菌株和chlororespirans D.菌株的内在选择标记。含有vanI的基因簇在系统发育上与土壤和肠道细菌中描述的基因簇只有较远的亲缘关系，但与放线菌门中发现的万古霉素耐药基因簇相反，放线菌门中包括几种产生万古霉素的细菌。它缺乏vanH同源物，编码d -乳酸脱氢酶，以前认为总是存在于万古霉素耐药基因簇中。vanH在抗性基因簇外的位置可能阻碍了基因的水平转移。因此，d.h hafniense中的万古霉素耐药簇应该被视为一个新的簇，我们在这里以其独特的d-Ala-d-Lac连接酶命名vanI。
The glycopeptide vancomycin was until recently considered a drug of last resort against Gram-positive bacteria. Increasing numbers of bacteria, however, are found to carry genes that confer resistance to this antibiotic. So far, 10 different vancomycin resistance clusters have been described. A chromosomal vancomycin resistance gene cluster was previously described for the anaerobic Desulfitobacterium hafniense Y51. We demonstrate that this gene cluster, characterized by its d-Ala-d-Lac ligase-encoding vanI gene, is present in all strains of D. hafniense, D. chlororespirans and some strains of Desulfosporosinus spp. This gene cluster was not found in vancomycin-sensitive Desulfitobacterium or Desulfosporosinus spp., and we show that this antibiotic resistance can be exploited as an intrinsic selection marker for Desulfitobacterium hafniense and D. chlororespirans. The gene cluster containing vanI is phylogenetically only distantly related with those described from soil and gut bacteria, but clusters instead with vancomycin resistance genes found within the phylum Actinobacteria that include several vancomycin-producing bacteria. It lacks a vanH homologue, encoding a D-lactate dehydrogenase, previously thought to always be present within vancomycin resistance gene clusters. The location of vanH outside the resistance gene cluster likely hinders horizontal gene transfer. Hence, the vancomycin resistance cluster in D. hafniense should be regarded a novel one that we here designated vanI after its unique d-Ala-d-Lac ligase.
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影响因子：
4.2
作者：
Kim SH;Harzman C;Davis JK;Hutcheson R;Broderick JB;Marsh TL;Tiedje JM
通讯作者：
Tiedje JM
影响因子：
4.9
作者：
Lebreton, Francois;Depardieu, Florence;Cattoir, Vincent
通讯作者：
Cattoir, Vincent
影响因子：
4.8
作者：
Hong, HJ;Hutchings, MI;Buttner, MJ
通讯作者：
Buttner, MJ
DOI：
10.1126/science.1220761
发表时间：
2012-08-31
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Forsberg KJ;Reyes A;Wang B;Selleck EM;Sommer MO;Dantas G
通讯作者：
Dantas G
影响因子：
3.2
作者：
Nonaka, H;Keresztes, G;Yukawa, H
通讯作者：
Yukawa, H