Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction.
Genome sequence of Desulfitobacterium hafniense DCB-2, a Gram-positive anaerobe capable of dehalogenation and metal reduction.
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DOI：
10.1186/1471-2180-12-21
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发表时间：
2012-02-08
期刊：
影响因子：
4.2
通讯作者：
Tiedje JM
中科院分区：
文献类型：
--
作者：
Kim SH;Harzman C;Davis JK;Hutcheson R;Broderick JB;Marsh TL;Tiedje JM
对革兰氏阳性、金属还原、脱盐的hafniense Desulfitobacterium hafniense DCB-2的基因组进行了测序，以了解其代谢能力、适应生理和调节机制，并与已测序的物种中系统发育最接近的菌株Desulfitobacterium hafniense Y51进行了比较。hafniense Desulfitobacterium DCB-2基因组由5279134 bp的环状染色体组成，预测基因为5042个。基因组含量和平行生理研究支持细胞固定N2和CO2、形成孢子和生物膜、减少金属以及在呼吸中使用各种电子受体（包括卤化有机化合物）的能力。该基因组包含7个还原脱卤酶基因和4个同源氮酶基因，但缺乏Nar呼吸硝酸盐还原酶系统。D. hafniense DCB-2基因组包含43个RNA聚合酶sigma因子基因，包括27个sigma-24亚基，59个双组分信号转导系统和约730个转运蛋白。此外，它还含有53种钼酸盐结合氧化还原酶的基因，19种富马酸还原酶的黄蛋白类似物，以及许多其他FAD/ fmn结合氧化还原酶的基因，证明了细胞在适应和还原能力方面的多功能性。与形成孢子的能力一起，固定二氧化碳的Wood-Ljungdahl途径和与氧耐受性相关的基因的存在增加了细胞在压力下生存的灵活性。D. hafniense DCB-2的基因组包含与其脱卤、金属还原、N2和CO2固定、厌氧呼吸、氧耐受性、孢子形成和生物膜形成能力一致的基因，这使该生物成为污染部位生物修复的潜在候选者。
The genome of the Gram-positive, metal-reducing, dehalorespiring Desulfitobacterium hafniense DCB-2 was sequenced in order to gain insights into its metabolic capacities, adaptive physiology, and regulatory machineries, and to compare with that of Desulfitobacterium hafniense Y51, the phylogenetically closest strain among the species with a sequenced genome. The genome of Desulfitobacterium hafniense DCB-2 is composed of a 5,279,134-bp circular chromosome with 5,042 predicted genes. Genome content and parallel physiological studies support the cell's ability to fix N2 and CO2, form spores and biofilms, reduce metals, and use a variety of electron acceptors in respiration, including halogenated organic compounds. The genome contained seven reductive dehalogenase genes and four nitrogenase gene homologs but lacked the Nar respiratory nitrate reductase system. The D. hafniense DCB-2 genome contained genes for 43 RNA polymerase sigma factors including 27 sigma-24 subunits, 59 two-component signal transduction systems, and about 730 transporter proteins. In addition, it contained genes for 53 molybdopterin-binding oxidoreductases, 19 flavoprotein paralogs of the fumarate reductase, and many other FAD/FMN-binding oxidoreductases, proving the cell's versatility in both adaptive and reductive capacities. Together with the ability to form spores, the presence of the CO2-fixing Wood-Ljungdahl pathway and the genes associated with oxygen tolerance add flexibility to the cell's options for survival under stress. D. hafniense DCB-2's genome contains genes consistent with its abilities for dehalogenation, metal reduction, N2 and CO2 fixation, anaerobic respiration, oxygen tolerance, spore formation, and biofilm formation which make this organism a potential candidate for bioremediation at contaminated sites.
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影响因子：
3.5
作者：
Christiansen, N;Ahring, BK;Diekert, G
通讯作者：
Diekert, G
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影响因子：
14.9
作者：
Gao, Feng;Zhang, Chun-Ting
通讯作者：
Zhang, Chun-Ting
影响因子：
14.9
作者：
Caspi R;Altman T;Dale JM;Dreher K;Fulcher CA;Gilham F;Kaipa P;Karthikeyan AS;Kothari A;Krummenacker M;Latendresse M;Mueller LA;Paley S;Popescu L;Pujar A;Shearer AG;Zhang P;Karp PD
通讯作者：
Karp PD
DOI：
10.1016/s0969-2126(99)80072-0
发表时间：
1999-05-01
期刊：
STRUCTURE WITH FOLDING & DESIGN
影响因子：
--
作者：
Garcin, E;Vernede, X;Fontecilla-Camps, JC
通讯作者：
Fontecilla-Camps, JC
影响因子：
3.6
作者：
Hensel, M;Hinsley, AP;Berks, BC
通讯作者：
Berks, BC