Novel leukaemogenic retroviruses isolated from cell line derived from spontaneous AKR tumour
Novel leukaemogenic retroviruses isolated from cell line derived from spontaneous AKR tumour
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从自发性 AKR 肿瘤细胞系中分离出新型致白血病逆转录病毒
DOI：
10.1038/292167a0
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发表时间：
1981
期刊：
影响因子：
64.8
通讯作者：
W. Haseltine
中科院分区：
文献类型：
--
作者：
F. Pedersen;R. Crowther;D. Tenney;A. Reimold;W. Haseltine
从AKR小鼠的白血病和白血病前期组织中分离的病毒在诱导胸腺淋巴瘤的能力上不同。来自白血病AKR小鼠胸腺1的总传代A病毒和从白血病前期和白血病AKR组织中分离的MCF病毒2，3在注射到新生AKR小鼠中时加速疾病发作3，4，而从妊娠中期开始在AKR小鼠中表达的内源性嗜亲性Akv病毒是非致白血病的3，5，6。这些病毒的基因组结构特征是什么？观察到致白血病MCF病毒的gp 70包膜糖蛋白含有亲嗜性和异嗜性区域7 -9，提出了gp 70基因的结构是致白血病活性的主要决定因素2的可能性。然而，克隆的、体外传代的Gross A病毒的致白血病分离株在基因组的gag-pol和gp 70编码区与非致白血病的Akv病毒非常相似10。Gross A和Akv病毒结构上的大部分差异位于3′-末端1，000个核苷酸内，这就提出了一种可能性，即gp 70基因以外靠近基因组3′-末端的序列编码致白血病活性的决定因素。由于Gross A病毒的传代史复杂，我们试图进一步分离未在其他动物中传代的致白血病AKR病毒，以确定它们是否与Gross A病毒相似。在这里，我们报告的新leukaemogenic病毒的分离和表征淋巴细胞系来自自发性AKR肿瘤。这些分离株在基因组的3′区类似于格罗斯A病毒。我们的研究结果表明，该区域编码的决定因素leukaemogenic效力和包膜糖蛋白的重大变化是不必要的leukaemogenic活性的这些病毒。
Viruses isolated from leukaemic and preleukaemic tissues of the AKR mouse differ in their ability to induce thymic lymphomas. The Gross passage A virus, which originates from the thymus of a leukaemic AKR mouse1, and the MCF viruses, isolated from preleukaemic and leukaemic AKR tissues2,3, accelerate the onset of disease on injection into newborn AKR mice3,4 whereas the endogenous ecotropic Akv virus, which is expressed in AKR mice from mid-gestation onward, is nonleukaemogenic3,5,6. What are the structural features of the genome that account for the leukaemogenic activity of these viruses? The observation that the gp70 envelope glycoprotein of the leukaemogenic MCF virus contain ecotropic and xenotropic regions7–9 raised the possibility that the structure of the gp70 gene was the major determinant of leukaemogenic activity2. However, a cloned, in vitro passaged, leukaemogenic isolate of the Gross A virus very closely resembles that of the nonleukaemogenic Akv virus in the gag–pol and gp70 coding regions of the genome10. Most of the differences in structure of the Gross A and Akv viruses are located within the 3′-terminal 1,000 nucleotides—this raises the possibility that sequences outside the gp70 gene which are proximal to the 3′ end of the genome encode determinants of leukaemogenic activity. Due to the complex passage history of the Gross A virus we sought to isolate further leukaemogenic AKR viruses that had not been passaged in other animals to determine whether or not they resembled the Gross A virus. Here we report the isolation and characterization of new leukaemogenic viruses from a lymphoid cell line derived from a spontaneous AKR tumour. These isolates resemble the Gross A virus in the 3′ region of the genome. Our results suggest that this region encodes determinants of leukaemogenic potency and that major changes in the envelope glycoprotein are not necessary for the leukaemogenic activity of these viruses.
水貂细胞焦点诱导鼠 C 型病毒的基因组分析：进展报告。
DOI：
10.1101/sqb.1980.044.01.138
发表时间：
1980
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Lung,ML;Hering,C;Hartley,JW;Rowe,WP;Hopkins,N
通讯作者：
Hopkins,N