Circulating Cell-Free DNA Level in Prediction of COVID-19 Severity and Mortality: Correlation of with Haematology and Serum Biochemical Parameters.
Circulating Cell-Free DNA Level in Prediction of COVID-19 Severity and Mortality: Correlation of with Haematology and Serum Biochemical Parameters.
复制标题
DOI：
10.1007/s12291-022-01082-4
复制
发表时间：
2023-04
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
冠状病毒病-19（COVID-19）中的淋巴细胞失调是与疾病严重程度和死亡率相关的主要因素。细胞凋亡导致循环中游离DNA（cfDNA）的积累。COVID-19具有异质性的临床病程。研究了cfDNA水平的作用，以评估COVID-19患者的严重程度和结局，并与其他实验室参数相关。当前的病例系列包括100名轻度COVID-19（MCOV-19）患者和106名重度COVID-19（SCOV-19）患者。使用SYBR绿色定量实时PCR通过β-肌动蛋白基因的扩增来定量血浆cfDNA水平。与MCOV-19中的219.8ng/ml（167.7-299.6）相比，SC 0 V-19中的CfDNA水平为706.7ng/ml（522.6-1258）显著更高。cfDNA水平在非存活者中显著高于存活者（p = 0.0001）。CfDNA显示与NLR、铁蛋白、LDH、降钙素原和IL-6显著相关。cfDNA对SCOV-19和MCOV-19的诊断敏感性和特异性分别为90.57%和80%。CfDNA在区分非存活者与存活者方面显示94.74%的灵敏度。CfDNA水平与COVID-19的其他实验室和炎症标志物呈显著正相关。CfDNA水平、NLR和其他参数可用于对COVID-19患者进行分层和监测，并预测死亡率。CfDNA可用于预测COVID-19的严重程度，具有更高的诊断灵敏度。
Lymphocyte dysregulation in coronavirus disease-19 (COVID-19) is a major contributing factor linked to disease severity and mortality. Apoptosis results in the accumulation of cell-free DNA (cfDNA) in circulation. COVID-19 has a heterogeneous clinical course. The role of cfDNA levels was studied to assess the severity and outcome of COVID-19 patients and correlated with other laboratory parameters. The current case series included 100 patients with mild COVID-19 (MCOV-19) and 106 patients with severe COVID-19 (SCOV-19). Plasma cfDNA levels were quantified using SYBR green quantitative real-time PCR through amplification of the β-actin gene. CfDNA level was significantly higher in SCOV-19 at 706.7 ng/ml (522.6–1258) as compared to MCOV-19 at 219.8 ng/ml (167.7–299.6). The cfDNA levels were significantly higher in non-survivor than in survivors (p = 0.0001). CfDNA showed a significant correlation with NLR, ferritin, LDH, procalcitonin, and IL-6. The diagnostic sensitivity and specificity of cfDNA in the discrimination of SCOV-19 from MCOV-19 were 90.57% & 80%, respectively. CfDNA showed a sensitivity of 94.74% in the differentiation of non-survivors from survivors. CfDNA levels showed a significant positive correlation with other laboratory and inflammatory markers of COVID-19. CfDNA levels, NLR, and other parameters may be used to stratify and monitor COVID-19 patients and predict mortality. CfDNA may be used to predict COVID-19 severity with higher diagnostic sensitivity.
登录
查看更多内容
影响因子：
5.3
作者：
Bronkhorst AJ;Ungerer V;Diehl F;Anker P;Dor Y;Fleischhacker M;Gahan PB;Hui L;Holdenrieder S;Thierry AR
通讯作者：
Thierry AR
影响因子：
7.3
作者：
Qun, Sen;Wang, Yulan;Zhou, Jian
通讯作者：
Zhou, Jian
影响因子：
168.9
作者：
Huang, Chaolin;Wang, Yeming;Cao, Bin
通讯作者：
Cao, Bin
影响因子：
28.2
作者：
Liu, Yuwei;Du, Xuebei;Zhao, Yan
通讯作者：
Zhao, Yan
影响因子：
1.8
作者：
Sakumoto, R;Shibaya, M;Okuda, K
通讯作者：
Okuda, K