Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B.
Proteomic response in Streptococcus gordonii DL1 biofilm cells during attachment to salivary MUC5B.
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DOI：
10.1080/20002297.2021.1967636
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发表时间：
2021
影响因子：
4.5
通讯作者：
Wickström C
中科院分区：
文献类型：
--
作者：
Robertsson C;Svensäter G;Blum Z;Jakobsson ME;Wickström C
唾液粘蛋白MUC5B似乎促进牙齿生物膜中的生物多样性，从而促进口腔健康，例如，通过在需要合作从复合糖蛋白释放营养素的各种微生物物种中诱导协同的“粘液溶解”活性。了解早期定植者如何与宿主唾液蛋白相互作用是更好地了解脓疱性有害口腔生物膜成熟的必要条件，并可为生物膜生理学提供关键见解。早期的口腔定植菌戈登链球菌DL1在无涂层、MUC5B或低密度唾液蛋白（LDP）涂层表面的生物膜流动池系统中进行了无张力生长。使用基于定量质谱的工作流程提取和分析细菌细胞蛋白，并鉴定差异表达的蛋白质。总体而言，S. gordonii DL1在不同条件下相似。鉴定了六种新的生物膜细胞蛋白和三种在所有生物膜培养物中均不存在的蛋白质。这些差异可能会提供深入了解适应生物膜生长在这个物种的机制。唾液MUC5B也在生物膜细胞蛋白质组中引起特异性反应。这些调节可能代表了这种粘蛋白可以促进细菌定殖的机制。口腔生物膜中的戈登氏菌
Salivary mucin MUC5B seems to promote biodiversity in dental biofilms, and thereby oral health, for example, by inducing synergistic ‘mucolytic’ activities in a variety of microbial species that need to cooperate for the release of nutrients from the complex glycoprotein. Knowledge of how early colonizers interact with host salivary proteins is integral to better understand the maturation of putatively harmful oral biofilms and could provide key insights into biofilm physiology. The early oral colonizer Streptococcus gordonii DL1 was grown planktonically and in biofilm flow cell systems with uncoated, MUC5B or low-density salivary protein (LDP) coated surfaces. Bacterial cell proteins were extracted and analyzed using a quantitative mass spectrometry-based workflow, and differentially expressed proteins were identified. Overall, the proteomic profiles of S. gordonii DL1 were similar across conditions. Six novel biofilm cell proteins and three planktonic proteins absent in all biofilm cultures were identified. These differences may provide insights into mechanisms for adaptation to biofilm growth in this species. Salivary MUC5B also elicited specific responses in the biofilm cell proteome. These regulations may represent mechanisms by which this mucin could promote colonization of the commensal S. gordonii in oral biofilms.
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影响因子：
3.1
作者：
Chaudhuri, Biswendu;Paju, Susanna;Scannapieco, Frank A.
通讯作者：
Scannapieco, Frank A.
影响因子：
3.1
作者：
Braza, Rezia Era;Silver, Aliyah B.;McIver, Kevin S.
通讯作者：
McIver, Kevin S.
DOI：
10.1099/13500872-140-12-3407
发表时间：
1994-12-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
BRADSHAW, DJ;HOMER, KA;BEIGHTON, D
通讯作者：
BEIGHTON, D
影响因子：
--
作者：
Cote, CK;Honeyman, AL
通讯作者：
Honeyman, AL
影响因子：
2.1
作者：
Liu, Yaling;Hu, Tao;Zhou, Xuedong
通讯作者：
Zhou, Xuedong