Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells
Protein expression profile in the differentiation of rat bone marrow stromal cells into Schwann cell-like cells
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大鼠骨髓基质细胞向雪旺细胞样细胞分化过程中的蛋白表达谱
DOI：
10.1007/s11427-009-0033-4
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Xu ZengLu
中科院分区：
文献类型：
--
作者：
Li WenTing;Sun HuaLin;Ding Fei;Gu XiaoSong;Xu ZengLu
在过去的十年中，越来越多的证据表明，骨髓基质细胞（MSCs）具有向神经谱系分化的潜力。许多研究报道，在实验条件下，MSC表现出形态学变化，并表达有限数量的神经蛋白。然而，尚未有关于MSC分化为雪旺细胞样细胞的蛋白质组学研究的报道。本研究从成年SD大鼠股骨和胫骨骨髓中分离骨髓间充质干细胞，并在体外进行诱导培养。采用双向凝胶电泳（2-DE）技术比较诱导分化前后MSCs蛋白质表达谱的变化。双向电泳结果显示，共获得792个蛋白质点，其中74个蛋白质点在分化前后发生了显著变化，其中43个蛋白质点表达上调，31个蛋白质点表达下调。我们通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）和数据库搜索分析了这74个点，发现它们可以分为不同的类别，包括细胞骨架和结构蛋白，生长因子，代谢蛋白，伴侣蛋白，受体蛋白，细胞周期蛋白，钙结合蛋白和其他蛋白。这些蛋白质还包括神经和神经胶质蛋白，如BDNF、CNTF和GFAP。本研究结果可为MSCs向雪旺细胞样细胞分化提供有价值的蛋白质组学信息。
During the last decade, increasing evidence suggested that bone marrow stromal cells (MSCs) have the potential to differentiate into neural lineages. Many studies have reported that MSCs showed morphological changes and expressed a limited number of neural proteins under experimental conditions. However, no proteomic studies on MSCs differentiated into Schwann cell-like cells have been reported. In this study, we isolated MSCs from adult Sprague-Dawley rat femur and tibia bone marrows and induced the cellsin vitrounder specific conditions. By using two-dimensional gel electrophoresis (2-DE), we compared the protein profiles of MSCs before and after induced differentiation. We obtained 792 protein spots in the protein profile by 2-DE, and found that 74 spots changed significantly before and after the differentiation using PDQuest software, with 43 up-regulated and 31 down-regulated. We analyzed these 74 spots by a matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF-MS) and by database searching, and found that they could be grouped into various classes, including cytoskeleton and structure proteins, growth factors, metabolic proteins, chaperone proteins, receptor proteins, cell cycle proteins, calcium binding proteins, and other proteins. These proteins also include neural and glial proteins, such as BDNF, CNTF and GFAP. The results may provide valuable proteomic information about the differentiation of MSCs into Schwann cell-like cells.
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影响因子：
5.2
作者：
Wislet-Gendebien, S;Hans, G;Rogister, B
通讯作者：
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影响因子：
4.8
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作者：
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影响因子：
2.1
作者：
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通讯作者：
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影响因子：
6.6
作者：
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通讯作者：
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